APT115 | Apo-BrdU™ Detection Kit

APT115
50 assays  
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      Overview

      Replacement Information

      Key Spec Table

      Key ApplicationsDetection Methods
      FCFluorescent
      Description
      Catalogue NumberAPT115
      Brand Family Chemicon®
      Trade Name
      • Apo-BrdU
      • Chemicon
      DescriptionApo-BrdU™ Detection Kit
      OverviewThe CHEMICON APO-BRDU™ Kit is a two color staining method for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry (Li et al. 1995). The kit contains the instructions and reagents required for measuring apoptosis in cells including; positive and negative control cells for assessing reagent performance; washing, reaction, and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), bromodeoxyuridine triphosphate (Br-dUTP), and fluorescein labeled anti-BrdU antibody for labeling DNA breaks and Propidium Iodide/RNase A solution for counter staining the total DNA.
      Materials Required but Not Delivered1. Flow Cytometer

      2. Distilled Water

      3. 1% (w/v) paraformaldehyde (methanol free) in PBS

      4. 70% (v/v) ethanol

      5. 37°C Water Bath

      6. Ice Bucket

      7. 12 x 75 mm flow cytometry test tubes

      8. Micro-pipettor
      References
      Product Information
      Components
      • The APO-BRDU™ Kit is shipped in one container and consists of two packages. One package is shipped at ambient temperature and should be stored at 2-8°C upon arrival. The other package is styrofoam containing frozen ice packs and the reagent contents should be stored at -20°C upon arrival. Note: Upon arrival store the reagents at the appropriate temperatures.
      • Reagent bottles have color coded caps to aid in their identification. Sufficient reagents are provided to process 60 cell suspensions including 5 mL positive and 5 mL negative control cell suspensions of approximately 1 x 106 cells per mL in 70% (v/v) ethanol. The control cells are derived from a human lymphoma cell line and have been fixed as described on page 6.
      • Positive Control Cells, Brown cap
      • Negative Control Cells, White cap
      • Wash Buffer, Blue cap
      • Reaction Buffer, Green cap
      • TdT Enzyme, Yellow cap
      • Br-dUTP, Violet cap
      • Rinsing Buffer, Red cap
      • Fluorescein~PRB-1 mAb, Orange cap
      • PI/RNase Staining Buffer, Amber bottle
      Detection methodFluorescent
      Applications
      ApplicationThe kit measures apoptosis in cells.
      Key Applications
      • Flow Cytometry
      Biological Information
      Species Reactivity
      • All
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsPrecautions and Warnings

      1. The components of this kit are for Research Use only and are not intended for diagnostic procedures.

      2. Positive and negative control cells contain 70% (v/v) ethanol as a preservative; wash and reaction buffer contain sodium cacodylate (dimethylarsinic) as a buffer; rinsing and PI/RNase staining buffer contain 0.05% (w/v) sodium azide as a preservative. These materials are harmful if swallowed; avoid skin contact, wash immediately with water. See Material Safety Data Sheets.

      3. TdT Enzyme will not freeze at -20oC, because it is in 50% (v/v) glycerol solution. Upon warming the TdT enzyme solution, centrifuge the tube for 30 seconds to force all the liquid to the bottom of the tube.
      Packaging Information
      Material Size50 assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Sperm apoptosis in fresh and cryopreserved bull semen detected by flow cytometry and its relationship with fertility.
      Muhammad Anzar, Liwei He, Mary M Buhr, Thomas G Kroetsch, Karl P Pauls
      Biology of reproduction  66  354-60  2002

      Show Abstract
      11804948 11804948

      User Guides

      Title
      Apo-BRDU Flow Cytometry Detection Kit