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CC1052 Human Integrin α5β1 Protein, Triton X-100 Formulation

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Positive Control UniProt Number: P08648 Entrez Gene Number: NM_002205.2
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CC1052
25 µg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberCC1052
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionHuman Integrin α5β1 Protein, Triton X-100 Formulation
      OverviewIntegrin a5/b1 purified from smooth muscle and placental tissue under nondenaturing conditions by affinity chromatography on fibronectin-Sepharose™ (Belkin, 1990).



      Two main protein bands corresponding to a5 (145 kD) and beta1 (120 kD) subunits are seen in silver stained 7.5%

      polyacrylamide gels under nonreduced conditions. Minor protein band at the 210 kD corresponding to beta1 subunit disulfide dimmer, which is formed during electrophoresis and disappears after reduction.
      Alternate Names
      • VLA-5
      References
      Product Information
      PresentationPurified protein in 20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 2 mM MgCl2 containing 0.2% Triton X-100. No preservatives. Source material for the enclosed purified human proteins, Integrin alpha5beta1, has been tested for antibodies to HIV, HCV, and HbsAg and found to be negative by an approved test.
      Quality LevelMQ100
      Applications
      Key Applications
      • Positive Control
      Application NotesElectrophoresis and Immunoblotting control. Due to the existance of reduction sensitive sites within the integrin subunits, it is recommended that gels be run under non-reducing conditions for best results. Under non-reducing conditions, the alpha5 subunit will migrate at 155 kDa while the beta1 subunit will migrate at 110 kDa.

      Function: Liposomes containing Integrin alpha5/beta1 adhere to surfaces coated with fibronectin in an RGD-dependent fashion.
      Biological Information
      PurityNonreduced SDS-PAGE of 15 mg on 5-15% polyacrylamide slab gel and staining with Coomassie shows only two main bands at 110 kDa (beta1 subunit) and 155 kDa (alpha5 subunit). A weak band at 205 kDa is due to dimerization of the b1 subunit (Belkin et al., 1996).

      CONTAMINANT(S):

      None identified.
      SourceHuman smooth muscle and placental tissue.
      Entrez Gene Number
      Entrez Gene SummaryThe product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
      Gene Symbol
      • ITGA5
      • VLA-5
      • CD49e
      • VLA5A
      • FNRA
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P08648 # Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
      SIZE: 1049 amino acids; 114536 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with HPS5. Interacts with HIV-1 Tat.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      SIMILARITY: SwissProt: P08648 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -70°C in undiluted aliquots. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size25 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CC1052 04053252370304

      Documentation

      Human Integrin α5β1 Protein, Triton X-100 Formulation SDS

      Title

      Safety Data Sheet (SDS) 

      Human Integrin α5β1 Protein, Triton X-100 Formulation Certificates of Analysis

      TitleLot Number
      PURIFIED HUMAN INTEGRIN A5/B1 (VLA-5) 3759035
      PURIFIED HUMAN INTEGRIN a5/b1 (VLA-5) - 2088945 2088945
      PURIFIED HUMAN INTEGRIN a5/b1 (VLA-5) - 2199585 2199585
      PURIFIED HUMAN INTEGRIN a5/b1 (VLA-5) - 2272727 2272727
      PURIFIED HUMAN INTEGRIN α5/β1 (VLA-5) 3110171
      PURIFIED HUMAN INTEGRIN α5/β1 (VLA-5) - 3539761 3539761
      PURIFIED HUMAN INTEGRIN α5/β1 - 3220379 3220379
      PURIFIED HUMAN INTEGRIN α5/β1 -2638908 2638908
      PURIFIED HUMAN INTEGRIN α5/β1 -2755740 2755740