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CP01 Anti-Actin (Ab-1) Mouse mAb (JLA20)

Anti-Actin (Ab-1), mouse monoclonal, clone JLA20, recognizes the ~ 42 kDa α-, β-, and γ isoforms of actin. It is validated for Western blotting and immunofluorescence.

Anti-Actin (Ab-1) Mouse mAb (JLA20) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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CP01
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
A Broad Range Of SpeciesMMonoclonal Antibody

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      CP01-1EA
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          Description
          OverviewRecognizes the ~42 kDa α-, β-, and γ isoforms of actin. Useful as a positive control antibody.
        • Antibody Target Gene Symbol: ACTA1, ACTB1, ACTG1
        • Target Synonym: AA959943, ACTA, Acta-2, Acts, Actsk-1, ALPHA SKELETAL ACTIN, ALPHA1 ACTIN, ASMA, CFTD, CFTD1, CFTDM, Fetal isoform of skeletal alpha-actin, G-actin, MPFD, NEM1, NEM2, NEM3, SKA, SKELETAL ALPHA-ACTIN, SKELETAL MUSCLE ACTIN
        • Entrez Gene Name: actin, alpha 1, skeletal muscle
        • Hu Entrez ID: 58, 71 (Related Antibodies: CP64)
        • Mu Entrez ID: 11459 ;-; 11465
        • Rat Entrez ID: 29437 ;-; 287876
          		•
          Catalogue NumberCP01
          Brand Family Calbiochem®
          References
          ReferencesLin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp.
          Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769.
          Lin, J.J. 1981. Proc. Natl. Acad. Sci. 78, 2335.
          Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701.
          Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.
          Product Information
          FormLiquid
          Formulation100 µl ascites diluted in 5 mM sodium phosphate buffer, pH 7.5 and 5 µg anti-mouse IgM, HRP conjugate supplied at 100 µg/ml in 0.05 M sodium phosphate, 50% glycerol.
          Positive controlAll animal cell lines
          Preservative≤0.1% sodium azide (actin antibody only, CP01-100UL)
          Quality LevelMQ100
          Applications
          Application ReferencesImmunoblotting Thomas, W.D., et al. 2000. J. Immunol. 165, 5612.
          Key Applications Immunoblotting (Western Blotting)
          Immunofluorescence
          Application NotesImmunoblotting (1:5000-1:20000)
          Immunofluorescence (1:10000, see application references)

          Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)
          Application CommentsActin is found in all animal cells. Actin monomers have a molecular weight of ~42 kDa. For best results, use the anti-mouse IgM HRP conjugate supplied with the anti-actin antibody. Useful for normalizing protein loading. Antibodies should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenchicken cytoskeletal proteins
          ImmunogenChicken
          CloneJLA20
          HostMouse
          IsotypeIgM
          Species Reactivity
          • A Broad Range Of Species
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot ascites and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Catalogue Number GTIN
          CP01-100UL 04053252046643
          CP01-1EA 07790788053697

          Documentation

          Anti-Actin (Ab-1) Mouse mAb (JLA20) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Actin (Ab-1) Mouse mAb (JLA20) Certificates of Analysis

          TitleLot Number
          CP01

          References

          Reference overview
          Lin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp.
          Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769.
          Lin, J.J. 1981. Proc. Natl. Acad. Sci. 78, 2335.
          Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701.
          Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.

          Brochure

          Title
          Antibody Sourcebookﾙ Sixth Edition
          Stem Cell Resource Brochure GBP

          Citations

          Title
        • Wayne D. Thomas, et al. (2000) TNF-Related Apoptosis-Inducing Ligand-Induced Apoptosis of Melanoma is Associated with Changes in Mitochondrial Membrane Potential and Perinuclear Clustering of Mitochondria. Journal of Immunology 165, 5612-5620.
          		•
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision29-October-2008 JSW
          ApplicationImmunoblotting (1:5000-1:20000)
          Immunofluorescence (1:10000, see application references)

          Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)
          DescriptionMouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells supplied as ascites. Recognizes the ~42 kDa α-, β- and γ-isoforms of actin. Supplied with goat anti-mouse IgM conjugated to horseradish peroxidase.
          BackgroundCytoskeletal proteins are found in highly organized arrays within the cytoplasm of higher eukaryotic cells. These proteins are intimately involved in functions such as cell and intracellular organelle movement, maintenance of cell shape and endocytosis. The cytoskeleton of most eukaryotic cells is comprised of at least three fibrous systems each with accessory proteins: the microfilaments, composed of actin; the microfibrils, composed of tubulin; and the intermediate filaments composed of vimentin. Monoclonal antibodies have been used to study the components of the cytoskeleton and their intracellular location, changes in the expression of these elements during differentiation, and the function of the cytoskeleton in living cells by microinjection of the antibodies.
          HostMouse
          Immunogen speciesChicken
          Immunogenchicken cytoskeletal proteins
          CloneJLA20
          IsotypeIgM
          Speciesa broad range of species
          Positive controlAll animal cell lines
          FormLiquid
          Formulation100 µl ascites diluted in 5 mM sodium phosphate buffer, pH 7.5 and 5 µg anti-mouse IgM, HRP conjugate supplied at 100 µg/ml in 0.05 M sodium phosphate, 50% glycerol.
          Preservative≤0.1% sodium azide (actin antibody only, CP01-100UL)
          CommentsActin is found in all animal cells. Actin monomers have a molecular weight of ~42 kDa. For best results, use the anti-mouse IgM HRP conjugate supplied with the anti-actin antibody. Useful for normalizing protein loading. Antibodies should be titrated for optimal results in individual systems.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot ascites and freeze (-20°C).
          Toxicity Multiple Toxicity Values, refer to MSDS
          ReferencesLin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp.
          Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769.
          Lin, J.J. 1981. Proc. Natl. Acad. Sci. 78, 2335.
          Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701.
          Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.
          Citation
        • Wayne D. Thomas, et al. (2000) TNF-Related Apoptosis-Inducing Ligand-Induced Apoptosis of Melanoma is Associated with Changes in Mitochondrial Membrane Potential and Perinuclear Clustering of Mitochondria. Journal of Immunology 165, 5612-5620.
          		•
          Application referencesImmunoblotting Thomas, W.D., et al. 2000. J. Immunol. 165, 5612.