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DR1094 Anti-PAX7 Mouse mAb (1E12)

This Anti-PAX7 Mouse mAb (1E12) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of PAX7.

Anti-PAX7 Mouse mAb (1E12) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Paired Box 7

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DR1094
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody

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DR1094-100UG
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      Description
      OverviewRecognizes the ~60 kDa PAX7 protein in NIH3T3 cells and human stomach tissue.
      Catalogue NumberDR1094
      Brand Family Calbiochem®
      SynonymsAnti-Paired Box 7
      Application Data
      Detection of mouse PAX7 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-PAX7 Mouse mAb (1E12) (Cat. No. DR1094) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of mouse PAX7 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human stomach tissue. Primary antibody: Anti-PAX7 Mouse mAb (1E12) (Cat. No. DR1094) (0.5 µg/ml). Detection: colorimetric (DAB).
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlNIH3T3 cells, Human stomach tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (0.5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 411-521 human PAX7, expressed as a GST fusion protein
      Clone1E12
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DR1094-100UG 04055977226133

      Documentation

      Anti-PAX7 Mouse mAb (1E12) SDS

      Title

      Safety Data Sheet (SDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-November-2010 RFH
      SynonymsAnti-Paired Box 7
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (0.5 µg/ml)
      Application Data
      Detection of mouse PAX7 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-PAX7 Mouse mAb (1E12) (Cat. No. DR1094) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of mouse PAX7 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human stomach tissue. Primary antibody: Anti-PAX7 Mouse mAb (1E12) (Cat. No. DR1094) (0.5 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~60 kDa PAX7 protein.
      BackgroundThis protein is a member of the paired box (PAX) family of transcription factors. Members of this family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These proteins play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Three transcript variants encoding different isoforms have been found for this gene.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 411-521 human PAX7, expressed as a GST fusion protein
      Clone1E12
      IsotypeIgG2a
      Specieshuman, mouse
      Positive controlNIH3T3 cells, Human stomach tissue
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review