Millipore Sigma Vibrant Logo

ES009-500ML p-NPP Single Reagent, Yellow, Alkaline Phosphatase Substrate (soluble)

500 ml p-NPP Single Reagent, Yellow, Alkaline Phosphatase Substrate (soluble) for ELISA.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: ELISA
View Products on Sigmaaldrich.com
ES009-500ML
500 mL  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Spec Table

      Key Applications
      ELISA
      Description
      Catalogue NumberES009-500ML
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      Descriptionp-NPP Single Reagent, Yellow, Alkaline Phosphatase Substrate (soluble)
      References
      Product Information
      HS Code3822 19 90
      Presentationp-NPP, a single component substrate in proprietary stabilizer.
      Color CodeYellow
      Applications
      Application500 ml p-NPP Single Reagent, Yellow, Alkaline Phosphatase Substrate (soluble) for ELISA.
      Key Applications
      • ELISA
      Application Notesp-Nitrophenyl phosphate (p-NPP) is the customary product utilized for the assay of alkaline phosphatase. It is usually supplied in tablet or powder form and is dissolved in an appropriate buffer immediately prior to assay. Liquid p-NPP concentrates are also available, but must also be diluted in buffer. CHEMICON, Inc. now provides p-NPP stabilized in diethanolamine (DEA) buffer and available for instant use. The DEA buffer contains Mg++ and Zn++ ions providing optimum activation of alkaline phosphatase labeled probes.

      This is an excellent substrate for phosphatase based ELISA assays.

      It produces a soluble end product which is yellow in color and reads at 405 nm to 410 nm. Both kinetic and end point determinations can be performed.
      Biological Information
      Physicochemical Information
      Quantitation Wavelength405 nm
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C for up to one year.
      Packaging Information
      Material Size500 mL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ES009-500ML 04053252409530

      Documentation

      p-NPP Single Reagent, Yellow, Alkaline Phosphatase Substrate (soluble) SDS

      Title

      Safety Data Sheet (SDS) 

      p-NPP Single Reagent, Yellow, Alkaline Phosphatase Substrate (soluble) Certificates of Analysis

      TitleLot Number
      p-NITROPHENYL PHOSPHATE (p-NPP) ALKALINE PHOSPHATASE SUBSTRATE - 2139964 2139964
      p-NITROPHENYL PHOSPHATE (p-NPP) ALKALINE PHOSPHATASE SUBSTRATE - 2141845 2141845
      p-NITROPHENYL PHOSPHATE (p-NPP) - 3276123 3276123
      p-NITROPHENYL PHOSPHATE (p-NPP) - 3536169 3536169
      p-NITROPHENYL PHOSPHATE (p-NPP) - 3782349 3782349
      p-NITROPHENYL PHOSPHATE (p-NPP) - 3826030 3826030
      p-NITROPHENYL PHOSPHATE (p-NPP) - 4043503 4043503
      p-NITROPHENYL PHOSPHATE (p-NPP) - 4043849 4043849
      p-NITROPHENYL PHOSPHATE (p-NPP) - 4120213 4120213
      p-NITROPHENYL PHOSPHATE (p-NPP) - 4198624 4198624
      Show more