ESG1106 | ESGRO® Recombinant Mouse LIF Protein

ESG1106
10⁶ units  1 mL
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      Overview

      Replacement Information

      Special Offers

      High Quality. Validated. Reliable.

      EmbryoMax PMEF feeder cells, Millipore's convenient solution for ES cell culture.
      Learn More

      Key Spec Table

      Key ApplicationsPurity
      CultThe active component mLIF has been shown to be >95% pure by SDS-PAGE. ESGRO® supplied 0.22 micron sterile filtered, and tested negative in mycoplasma tests.
      Description
      Catalogue NumberESG1106
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionESGRO® Recombinant Mouse LIF Protein
      OverviewThe ESGRO mouse LIF medium supplement is supplied in active units/mL for consistent performance from lot to lot. Each lot of ESGRO supplement undergoes stringent quality control testing to ensure reliable inhibition of mouse pluripotent stem cell differentiation. ESGRO mouse LIF supplement may be used for feeder based and feeder free culture of mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells.
      Alternate Names
      • Murine LIF
      • Mouse LIF
      • mLIF
      Background InformationMouse Leukemia Inhibitory Factor (LIF, mLIF) promotes self-renewal and long-term maintenance of pluripotent mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells by suppressing spontaneous differentiation.
      References
      Product Information
      FormatPurified
      PresentationESGRO® Supplement is supplied in liquid form as 106 Units in 1.0 mL of phosphate buffered saline with 1% w/v bovine serum albumin BSA as a carrier for stability.
      Applications
      ApplicationESGRO Leukemia Inhibitory Factor (LIF) supplement for mouse ES cell culture. Each vial contains 10^6 units/ml
      Key Applications
      • Stem Cell Culture
      Application NotesApplications for ESGRO® Supplement include use as a reagent for the in vitro maintenance of the pluripotential phenotype of murine ES cells.

      SUGGESTED PROTOCOLS:

      ES Cells: In D3 and MBL-1 pluripotential ES cell cultures it is routinely found that 1000 U of ESGRO® Supplement per 1.0 mL of tissue culture media is required to maintain ES cells with a stem cell phenotype. Similar concentrations of mLIF have also been used for germline transmission of genetically altered ES cells (E3).

      At the recommended concentration 107 units of ESGRO® Supplement is sufficient for 10.0 L of tissue culture media and 106 units of ESGRO® Supplement is sufficient for 1.0 L of tissue culture media.
      Biological Information
      Concentration1 million units/1 mL
      PurityThe active component mLIF has been shown to be >95% pure by SDS-PAGE. ESGRO® supplied 0.22 micron sterile filtered, and tested negative in mycoplasma tests.
      Specific ActivityESGRO® Supplement is assessed both on mouse embryonic stem cells (D3 & MBL-1) and on murine M1 myeloid leukemic cells(4). A standard of 50 Units is defined as the concentration of ESGRO® Supplement in 1.0mL of tissue culture medium that induces the differentiation of 50% of M1 colonies(4). 10<sup>6</sup> units is equivalent to approximately 10 μg of pure protein, and is sufficient to treat 1L of ES cell culture media. <br /><br />Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mL Murine myeloid leukemic, M1 Assay: Specific Activity >/= 10<sup>8</sup> units/mg
      Entrez Gene Number
      Entrez Gene SummaryLeukaemia inhibitory factor is a cytokine that induces macrophage differentiation. Neurotransmitters and neuropeptides, well known for their role in the communication between neurons, are also capable of activating monocytes and macrophages and inducing chemotaxis in immune cells. LIF signals through different receptors and transcription factors. LIF in conjunction with BMP2 acts in synergy on primary fetal neural progenitor cells to induce astrocytes.
      Gene Symbol
      • LIF
      • Emfilermin
      • CDF
      • MLPLI
      • HILDA
      • D-FACTOR
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P15018 # LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
      SIZE: 202 amino acids; 22008 Da
      SUBCELLULAR LOCATION: Secreted.
      SIMILARITY: SwissProt: P15018 ## Belongs to the LIF/OSM family.
      Media FormLiquid
      Stem Cell Type
      • Mouse Embryonic Stem Cells
      • Induced Pluripotent Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      • LABEL LICENSE - USE RESTRICTED TO RESEARCH USE ONLY

        Leukemia Inhibitory Factor ("LIF") is protected by US Patents No. 5,443,825 and 5,750,654 and Canada Patent No. 1,341,581 and 1,341,469 owned by AMRAD Corporation Ltd. and licensed exclusively to Millipore for research uses. The use of this product is limited for research uses only and not for any commercial purposes, such as making or developing other research products from this product or use of this product as a component in a research kit. This product is not to be used in humans or for any diagnostic purposes.

        The purchaser may refuse to accept the conditions of this label license by returning this product unopened and will receive a full refund of the purchase price paid.

        For-profit entities or commercial research entities purchasing this product shall be required to execute a separate commercial license with Millipore within three months of purchase. Researchers may not transfer this product or its derivatives to researchers performing commercial research at other Nonprofit Organizations, or to For-Profit Organizations, without the express written consent of Millipore, and without those entities having an express license from Millipore for the use of the product. Other than as specifically provided herein, a transferee of this product shall have no right to transfer this product, or its derivatives to any other person or entity.
      Storage and Shipping Information
      Storage ConditionsESGRO® Supplement is shipped at ambient temperature. A cold pack may be included for customer relations, however it is truly not needed. Extensive stability tests have been performed with ESGRO® Supplement during development. This product is stable for at least 18 months from the date of manufacture, in the concentrated form or diluted in sterile tissue culture media, with no loss of activity on ES cells. For long term storage it is recommended that ESGRO® Supplement be stored at 4°C. Freeze-thawing will reduce potency. It is recommended that prior to use, ESGRO® Supplement should be diluted in sterile tissue culture media and aliquoted to a convenient concentration, then stored at 4°C. Freeze-thawing should be avoided. Product is stable for a minimum of 7 days at 37°C, 5% CO2 incubator during the culture of ES cells.
      Packaging Information
      Material Size10⁶ units
      Material Package1 mL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      Colony Picking
      ES Cell Culture using ESGRO Medium Supplement
      Electroporation of ES Cells
      Harvesting and DNA Preparation
      Karyotyping ES Cells
      Selection of ES Cells

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      ESGRO#174; Mouse LIF Medium Supplement - 1993949 1993949
      ESGRO#174; Mouse LIF Medium Supplement - 2179117 2179117
      ESGRO#174; Mouse LIF Medium Supplement - 2197551 2197551
      ESGRO#174; Mouse LIF Medium Supplement Cell Culture Supplement - 2073737 2073737
      ESGRO#174; Supplement (106 Units) - 7240-102E 7240-102E
      ESGRO#174; Supplement (106 Units) - DAM1770435 DAM1770435
      ESGRO#174; Supplement (106 Units) - DAM1797304 DAM1797304
      ESGRO#174; Supplement (106 Units) - DAM1797314 DAM1797314
      ESGRO#174; Supplement (106 Units) - JBC1872219 JBC1872219
      ESGRO® Mouse LIF Medium -2592751 2592751

      References

      Reference overviewApplicationSpeciesPub Med ID
      Modulating Glypican4 suppresses tumorigenicity of embryonic stem cells while preserving self-renewal and pluripotency.
      Fico, Annalisa, et al.
      Stem Cells, 30: 1863-74 (2012)  2012

      Show Abstract
      22761013 22761013
      A conditional knockout resource for the genome-wide study of mouse gene function
      Skarnes W. C. et al.
      Nature  474(7351)  337-342  2011

      Show Abstract
      21677750 21677750
      High-throughput mapping of protein occupancy identifies functional elements without the restriction of a candidate factor approach.
      Ferraris, L, et al.
      Nucleic Acids Res., 39: e33 (2011)  2011

      Show Abstract
      Immunoblotting (Western)Mouse21169336 21169336
      Optimization of Protocols for Derivation of Mouse Embryonic Stem Cell Lines from Refractory Strains, Including the Non Obese Diabetic Mouse.
      Davies T. J. & Fairchild P. J.
      Stem Cells Dev.  Nov.  2011

      Show Abstract
      21933027 21933027
      Proliferating versus differentiating stem and cancer cells exhibit distinct midbody-release behaviour.
      Ettinger A. W. et al.
      Nat. Commun.  2  503  2011

      Show Abstract
      22009035 22009035
      Dynamic regulation of 5-hydroxymethylcytosine in mouse ES cells and during differentiation.
      Ficz G. et al.
      Nature  473(7347)  398-402  2011

      Show Abstract
      21460836 21460836
      Combinatorial binding of transcription factors in the pluripotency control regions of the genome.
      Ferraris, Luciana, et al.
      Genome research, (2011)  2011

      Show Abstract
      Cell CultureMouse21527551 21527551
      Systematic delineation of optimal cytokine concentrations to expand hematopoietic stem/progenitor cells in co-culture with mesenchymal stem cells.
      Andrade, Pedro Z, et al.
      Mol Biosyst, 6: 1207-15 (2010)  2010

      Show Abstract
      20424784 20424784
      Using small molecules to improve generation of induced pluripotent stem cells from somatic cells
      Desponts C. & Ding S.
      Methods Mol. Biol.  636  207-218  2010

      Show Abstract
      20336525 20336525
      Short RNAs are transcribed from repressed polycomb target genes and interact with polycomb repressive complex-2.
      Kanhere, Aditi, et al.
      Mol. Cell, 38: 675-88 (2010)  2010

      Show Abstract
      20542000 20542000

      Brochure

      Title
      Murine Embryonic Stem Cell Culture Procedures & Protocols

      Technical Info

      Title
      Stem Cell Testing of PES Membrane Containing Stericup Filters
      The Genetically Modified Mouse: An Indispensable Tool for Disease Modeling

      Data Sheet

      Title
      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation
      STEMCCA Lentivirus Reprogramming Kits

      FAQ

      QuestionAnswer
      Cause of Differation: Incubator settingsRecommendation: Ensure that the CO2 incubator readings are correct at 37oC and 5% CO2.
      Cause of Differation: Concentration of ES cellsRecommendation: Low confluency of ES cells can result in differentiation. ES cells should be plated at a minimum density of 1x106 cells / 100mm dish. Refer to the attached figures for illustrations of ES cell confluency. Refer to figs. 1, 2, 3, 4, and 5.
      Cause of Differation: GelatinRecommendation: It is preferable to use cell culture grade gelatin at all times (even if using feeder layers) as the gelatin minimises surface differences on the tissue culture plates.
      Cause for the lack of chimeras: ES cell passage numberRecommendation: In general; the best chimeras are generated from low passage number ES cells. If cell lines have been cultured for a high number of passages, it is recommended to go back to a lower passage frozen stock.
      Cause for the lack of chimeras: Time taken prior to microinjectionRecommendation: It is recommend that ES cells be microinjected as soon as possible after removing them from the tissue culture plates, and not left on ice or room temperature for extended periods.
      Why are my mouse embryonic stem cells differentiating and what preventative measures do you recommend?Recommendation: Regular use of Penicillin / Streptomycin in media can often mask a low level contamination with agents such as mycoplasma. It is recommended to have your cell lines tested for mycoplasma on a periodic basis.
      Why are my mouse embryonic stem cells differentiating and what preventative measures do you recommend?Recommendation: Regular use of Penicillin / Streptomycin in media can often mask a low level contamination with agents such as mycoplasma. It is recommended to have your cell lines tested for mycoplasma on a periodic basis.
      Why are my mouse embryonic stem cells differentiating and what preventative measures do you recommend?Recommendation: Regular use of Penicillin / Streptomycin in media can often mask a low level contamination with agents such as mycoplasma. It is recommended to have your cell lines tested for mycoplasma on a periodic basis.

      Newsletters / Publications

      Title
      Cellutions Newsletter: 2007, Volume 1
      Cellutions Newsletter: 2008, Volume 1
      Cellutions Newsletter: 2008, Volume 2
      Cellutions Newsletter: 2008, Volume 3