EGLP-35K | Glucagon Like Peptide-1 (Active) ELISA

EGLP-35K
96-well plate  
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      Overview

      Replacement Information

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      Glucagon Like Peptide-1H, M, RELISAFluorescent
      Description
      Catalogue NumberEGLP-35K
      DescriptionGlucagon Like Peptide-1 (Active) ELISA
      Background InformationGLP-1 is secreted from gut endocrine cells in response to nutrient ingestion and plays multiple roles in metabolic homeostasis.
      References
      Product Information
      Detection methodFluorescent
      Precision, %
      • Inter-assay: 8 ± 4.8
      • Intra-assay: 7.4 ± 1.1
      Applications
      ApplicationThis Glucagon Like Peptide-1 (Active) ELISA is used to measure & quantify Glucagon Like Peptide-1 levels in Metabolism & Endocrine research.
      Key Applications
      • ELISA
      Application NotesOvernight incubation at 4°C, followed by 2 hours 30 minutes at room temperature Sample volume 100 μl serum, plasma or tissue culture medium
      Biological Information
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Analytes Available
      • Glucagon Like Peptide-1
      Cross ReactivityGLP-1 is conserved in almost all mammals. This kit may be used to mearure in such species (e.g. rat, mouse, primates, etc.)
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon.
      Gene Symbol
      • GCG
      • GRPP
      • GLP1
      • GLP2
      • glucagon
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01275 # Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
      SIZE: 180 amino acids; 20909 Da
      SUBCELLULAR LOCATION: Secreted.
      TISSUE SPECIFICITY: Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain.
      PTM: Proglucagon is post-translationally processed in a tissue- specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.
      SIMILARITY: SwissProt: P01275 ## Belongs to the glucagon family.
      MISCELLANEOUS: In the glucagon antagonist, His-53 and Phe-58 are missing. This antagonist has been successfully utilized to reduce glucose concentration in vivo.
      Accuracy
      • 86.9 ± 5.2%
      Physicochemical Information
      Standard Curve Range
      • 2–100 pM
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96-well plate
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      Protocol: Glucagon-Like Peptide-1 (Active) ELISA KIT - 96-Well Plate

      SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Fructose stimulates GLP-1 but not GIP secretion in mice, rats, and humans.
      Rune E. Kuhre , Fiona M. Gribble , Bolette Hartmann , Frank Reimann , Johanne A. Windeløv , Jens F. Rehfeld , Jens J. Holst
      Am J Physiol Gastrointest Liver Physiol. 2014 Apr 1;306(7):G622-30.  2014

      Show Abstract
      24525020 24525020
      Circulating Glucagon-Like Peptide-1 (Glp-1) Inhibits Eating In Male Rats By Acting In The Hindbrain And Without Inducing Avoidance
      Mukesh Punjabi, Myrtha Arnold, Elisabeth Rüttimann, Mariana Graber, Nori Geary, Gustavo Pacheco-López, and Wolfgang Langhans
      Endocrinology. 2014 May;155(5):1690-9.  2014

      Show Abstract
      24601880 24601880
      Glucagon-Like Peptide-1 Secretory Function As An Independent Determinant Of Blood Pressure: Analysis In The Tanno-Sobetsu Study
      Mayumi Yoshihara, Hiroshi Akasaka, Hirofumi Ohnishi, Takayuki Miki, Tetsuaki Furukawa, Satoshi Yuda, Shigeyuki Saitoh, Tetsuji Miura
      PLoS One. 2013 Jul 2;8(7):e67578. doi: 10.1371/journal.pone.0067578.  2013

      Show Abstract
      23844037 23844037
      Evidence For Paracrine/Autocrine Regulation Of Glp-1-Producing Cells
      Camilla Kappe , Qimin Zhang , Jens J. Holst , Thomas Nyström , Åke Sjöholm
      Am J Physiol Cell Physiol. 2013 Nov 15;305(10):C1041-9.  2013

      Show Abstract
      23986202 23986202
      Effects Of Sleep Fragmentation On Appetite And Related Hormone Concentrations Over 24 H In Healthy Men
      Hanne K. J. Gonnissen, Rick Hursel, Femke Rutters, Eveline A. P. Martens and Margriet S. Westerterp-Plantenga
      Br J Nutr. 2013 Feb 28;109(4):748-56.  2013

      Show Abstract
      22682471 22682471
      Meal-contingent intestinal lymph sampling from awake, unrestrained rats.
      Myrtha Arnold,Yunting Dai,Patrick Tso,Wolfgang Langhans
      American journal of physiology. Regulatory, integrative and comparative physiology  302  2012

      Show Abstract
      22513747 22513747
      GLP-1 Receptor Antagonist Exendin-(9-39) Elevates Fasting Blood Glucose Levels in Congenital Hyperinsulinism Owing to Inactivating Mutations in the ATP-Sensitive K+ Channel.
      Andrew C Calabria,Changhong Li,Paul R Gallagher,Charles A Stanley,Diva D De Le
      Diabetes  61  2012

      Show Abstract
      22855730 22855730
      Rectal taurocholate increases L cell and insulin secretion, and decreases blood glucose and food intake in obese type 2 diabetic volunteers.
      T E Adrian,S Gariballa,K A Parekh,S A Thomas,H Saadi,J Al Kaabi,N Nagelkerke,B Gedulin,A A Young
      Diabetologia  55  2012

      Show Abstract
      22696033 22696033
      Regular consumption of pulses for 8 weeks reduces metabolic syndrome risk factors in overweight and obese adults.
      R C Mollard,B L Luhovyy,S Panahi,M Nunez,A Hanley,G H Anderson
      The British journal of nutrition  108 Suppl 1  2012

      Show Abstract
      22916807 22916807
      Reduced levels of active GLP-1 in patients with cystic fibrosis with and without diabetes mellitus.
      Magnus Hillman,Leif Eriksson,Lena Mared,Karin Helgesson,Mona Landin-Olsson
      Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society  11  2012

      Show Abstract
      22138561 22138561

      Brochure

      Title
      Custom Immunoassay Menu of Services
      ELISAs, RIAs & GyroMark HT Assays

      Technical Info

      Title
      96 well Template 2 Up
      96 well Template Single
      Paper: Roux en Y gastric bypass hypoglycemia resolves with gastric feeding or reversal: Confirming a non-pancreatic etiology
      Quality Control Ranges: GLP-1 (Active) ELISA

      Data Sheet

      Title
      Full Service Custom-built Assays

      Posters

      Title
      Multiplex Assays of Fifteen Human Complement Factors

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits