03-190 HDAC1 (Hist-tag) human recombinant

50 µg  
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      Replacement Information

      Key Spec Table

      Key ApplicationsSpecies
      Enzyme AssaysHuman
      Catalogue Number03-190
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionHDAC1 (Hist-tag) human recombinant
      OverviewHDAC1 (Hist-tag) human recombinant produced in insect cells
      Background InformationHDAC1 promotes transcriptional repression by deacetylating lysine ε-amino groups in histone N-terminal tails, a function frequently carried out in association with multi-protein transcription repression complexes such as NuRD3, Sin34 and CoREST6. Ubiquitously expressed in human tissues, HDAC1-containing complexes (at least class I) appear to contribute the greater part of deacetylase activity in HeLa nuclear extracts. Aside from its interaction with co-repressors, HDAC1 activity may be regulated by post-translational modifications, such as phosphorylation and sumoylation, or binding to the inhibitor maspin, a tumor-suppressive serpin homolog. Although originally described as a "histone deacetylase," HDAC1 has been shown to catalyze the regulatory deacetylation of non-histone proteins, including p53. Overexpression of HDAC1 has been found in various types of cancer. HDAC inhibitors (HDACi) have shown considerable promise as anti-cancer agents.
      Product Information
      PresentationIn liquid containing 50 mM Tris, pH 8.0, 138 mM NaCl and 10% glycerol. Frozen solution.
      ApplicationHDAC1 (Hist-tag) human recombinant produced in insect cells.
      Key Applications
      • Enzyme Assays
      Application NotesHDAC deacetylase assays
      Biological Information
      PurityPurity >50% by SDS-PAGE
      Specific Activity>1U/µg. 1U = 1 pmol/min at 37°C using a standard flourometric activity assay to measure deacetylation activity
      Molecular Weight~55 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -80°C. The enzyme is stable on ice for 30 to 60 min, but may lose activity with dilution and/or prolonged storage on ice. It is recommended that thawing and addition of the enzyme to assay wells be done within as short a time as possible before start of the assay and that the thawed enzyme be kept on ice throughout. Best performance will be obtained by adding the chilled, undiluted enzyme directly to pre-warmed buffer and proceeding immediately to the addition of pre-warmed substrate. The remaining, unused enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the HDAC1 into separate tubes before storing at -80°C.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information

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      Life Science Research > Proteins and Enzymes > Other Enzymes