EZGRT-89K | Human Ghrelin (total) ELISA

EZGRT-89K
96-well strip plate  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      Ghrelin (Total)HELISAFluorescent, Colorimetric
      Description
      Catalogue NumberEZGRT-89K
      DescriptionHuman Ghrelin (total) ELISA
      Background InformationCirculating ghrelin is a multifunctional hormone secreted primarily by the stomach.
      References
      Product Information
      Detection methodFluorescent Colorimetric
      Precision, %
      • Inter-assay: Serum: 6.2–7.8% Plasma: 5.2–7.7%
      • Intra-assay: Serum: 0.9–1.3% Plasma: 1.1–1.9%
      Applications
      ApplicationThis Human Ghrelin (total) ELISA is used to measure & quantify Ghrelin (total) levels in Metabolism & Endocrine & Neuroscience research.
      Key Applications
      • ELISA
      Application NotesRoom temperature, 3 hour assay, 20 μL sample size (serum or plasma)
      Biological Information
      SpecificityHuman Ghrelin (active) 80% Des-Octanoyl Human Ghrelin 100% Canine Ghrelin (active) 70% Porcine Ghrelin (active) not detectable Motilin Related Peptide (Human, Rat/Mouse) not detectable PYY 3-36 (Human, Mouse, Porcine) not detectable NPY (Human/Rat)
      Species Reactivity
      • Human
      Analytes Available
      • Ghrelin (Total)
      Entrez Gene Number
      Entrez Gene SummaryGhrelin is an endogenous ligand for the growth hormone secretagogue receptor (GHSR; MIM 601898) and is involved in regulating growth hormone (GH; MIM 139250) release. Ghrelin is derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 (MIM 602886) and is involved in satiety and decreased food intake.[supplied by OMIM]
      Gene Symbol
      • GHRL
      • ghrelin
      • obestatin
      • MTLRP
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UBU3 # Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility (By similarity).
      SIZE: 117 amino acids; 12911 Da
      SUBCELLULAR LOCATION: Secreted.
      TISSUE SPECIFICITY: Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
      PTM: O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28- C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28- C10:1, may be modified with a singly unsaturated carboxylic acid. & Amidation of Leu-98 is essential for obestatin activity (By similarity).
      SIMILARITY: SwissProt: Q9UBU3 ## Belongs to the motilin family.
      Accuracy
      • Serum: (Active Ghrelin) 88–91%
      • (Des-octanoyl Ghrelin) 101–107%
      • Plasma: (Active Ghrelin) 84–87%
      • (Des-octanoyl Ghrelin) 112–119%
      Physicochemical Information
      Sensitivity
      • 30 pg/mL (20 µL sample size)
      Linearity of DilutionSerum: 95.7–104.7% Plasma: 95–117%
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Availability by Geography
      • This product is not available for sale in Japan.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96-well strip plate
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      Human Ghrelin (Total) ELISA Kit

      SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Changes in glucose homeostasis after Roux-en-Y gastric bypass surgery for obesity at day three, two months, and one year after surgery: role of gut peptides.
      Falkén Y, Hellström PM, Holst JJ, Näslund E
      The Journal of clinical endocrinology and metabolism  96  2227-35. Epub 2011 May 4.  2011

      21543426 21543426
      Immunohistochemical and quantitative analysis of ghrelin in Syzygium aromaticum.
      Aydin S, Dagli AF, Ozkan Y, Kendir Y, Sahin I, Aksoy A, Ozercan IH.
      Cell biology international  35  437-41  2011

      Show Abstract
      21029042 21029042
      The fermentable fibre inulin increases postprandial serum short-chain fatty acids and reduces free-fatty acids and ghrelin in healthy subjects.
      Tarini J, Wolever TM
      Applied physiology, nutrition, and metabolism = Physiologie  35  9-16.  2010

      Show Abstract
      20130660 20130660
      Alteration in chromogranin A, obestatin and total ghrelin levels of saliva and serum in epilepsy cases.
      Ersel Dag,Suleyman Aydin,Yusuf Ozkan,Fazilet Erman,Adile Ferda Dagli,Mehtap Gurger
      Peptides  31  2010

      Show Abstract
      20172008 20172008

      Brochure

      Title
      Custom Immunoassay Menu of Services
      ELISAs, RIAs & GyroMark HT Assays

      Technical Info

      Title
      96 well Template 2 Up
      96 well Template Single
      Quality Control Ranges: Human Total Ghrelin ELISA KIT - 96-Well Plate

      Data Sheet

      Title
      Full Service Custom-built Assays

      Posters

      Title
      Multiplex Assays of Fifteen Human Complement Factors

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      EZGRT-89BK Human Ghrelin (total) ELISA- Bulk Show Pricing & Availability

      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits