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IF02L Anti-Desmin (Ab-1) Mouse mAb (DE-B-5)

Anti-Desmin (Ab-1) Mouse mAb (DE-B-5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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IF02L
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~53 kDa Desmin protein in muscle cells and tissue. Does not cross-react with other classes of intermediate filaments.
      Catalogue NumberIF02L
      Brand Family Calbiochem®
      References
      ReferencesDebus, E., et al. 1983. EMBO J. 2, 2305.
      Geisler, N., et al. 1982. Cell 30, 277.
      Lazarides, E. 1982. Ann. Rev. Biochem. 51, 219.
      Osborn, M. and Weber, K., 1982. Cell 31, 303.
      Product Information
      FormLyophilized
      FormulationLyophilized from 20 mM NH₄HCO₃, 100 µg BSA.
      Negative controlEpithelial cells or tissue
      Positive controlMuscle cells or tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting, Immunocytochemistry, Frozen Sections, Paraffin Sections Droese, M., et al. 1984. Acta. Cytol. 28, 368. Osborn, M., et al. 1984. Cold Spring Harbor Lab., 191. Cross-Reactivity Altmannsberger, M., et al. 1985. Am. J. Pathol. 118, 85. Specificity Droese, M., et al. 1984. Acta. Cytol. 28, 368. Osborn, M., et al. 1984. Cold Spring Harbor Lab., 191. Debus, E., et al. 1983. EMBO J. 2, 2305. Osborn, M. and Weber, K. 1983. Lab. Invest. 48, 372. Altmannsberger, M., et al. 1982. Lab. Invest. 46, 520.
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Paraffin Sections
      Application NotesFrozen Sections (1-5 µg/ml, see application references)
      Immunoblotting (1-5 µg/ml, see application references)
      Immunocytochemistry (1-5 µg/ml, see application references)
      Paraffin Sections (1-10 µg/ml, heat pre-treatment required, see application references)
      Application CommentsThis antibody stains intermediate filaments found in skeletal, visceral and vascular smooth muscle cells, as well as those found in certain established cell lines such as the human rhabdomyosarcoma line (see application references). Desmin is also found in rhabdomyosarcoma biopsies, and is positively stained in smaller, round cells, which are difficult to judge histologically as rhabdomyosarcoma. Does not recognize other classes of intermediate filament proteins (see application references). Antibody should be titrated for optimal results in individual systems.
      Biological Information
      ImmunogenHuman
      CloneDE-B-5
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsWe recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Avoid freeze/thaw cycles. Antibody should be titrated for optimal results in individual systems.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      IF02L 0

      Documentation

      Anti-Desmin (Ab-1) Mouse mAb (DE-B-5) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Desmin (Ab-1) Mouse mAb (DE-B-5) Certificates of Analysis

      TitleLot Number
      IF02L

      References

      Reference overview
      Debus, E., et al. 1983. EMBO J. 2, 2305.
      Geisler, N., et al. 1982. Cell 30, 277.
      Lazarides, E. 1982. Ann. Rev. Biochem. 51, 219.
      Osborn, M. and Weber, K., 1982. Cell 31, 303.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-August-2007 RFH
      ApplicationFrozen Sections (1-5 µg/ml, see application references)
      Immunoblotting (1-5 µg/ml, see application references)
      Immunocytochemistry (1-5 µg/ml, see application references)
      Paraffin Sections (1-10 µg/ml, heat pre-treatment required, see application references)
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogent and fusing splenocytes with PAI mouse myeloma cells (see application references). Recognizes the ~53 kDa Desmin protein.
      BackgroundIntermediate (10 nm) filaments are present in, and provide information concerning the origin of, most vertebrate cells. At present, five major cell types can be distinguished by their intermediate filament components. These include epithelial cells (cytokeratins), neurons (neurofilaments), glial cells (GFA), skeletal, visceral and certain vascular smooth muscle cells (desmin) and various nonepithelial cells, including cells of mesenchymal origin (vimentin). Desmin (Ab-1) is a mouse monoclonal antibody, which identifies the desmin subclass of intermediate filaments in human tissues, specifically recognizing a site between amino acid residues 325-372.
      HostMouse
      Immunogen speciesHuman
      CloneDE-B-5
      IsotypeIgG₁
      Specieshuman
      Positive controlMuscle cells or tissue
      Negative controlEpithelial cells or tissue
      FormLyophilized
      FormulationLyophilized from 20 mM NH₄HCO₃, 100 µg BSA.
      PreservativeNone
      CommentsThis antibody stains intermediate filaments found in skeletal, visceral and vascular smooth muscle cells, as well as those found in certain established cell lines such as the human rhabdomyosarcoma line (see application references). Desmin is also found in rhabdomyosarcoma biopsies, and is positively stained in smaller, round cells, which are difficult to judge histologically as rhabdomyosarcoma. Does not recognize other classes of intermediate filament proteins (see application references). Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsWe recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Avoid freeze/thaw cycles. Antibody should be titrated for optimal results in individual systems.
      Toxicity Standard Handling
      ReferencesDebus, E., et al. 1983. EMBO J. 2, 2305.
      Geisler, N., et al. 1982. Cell 30, 277.
      Lazarides, E. 1982. Ann. Rev. Biochem. 51, 219.
      Osborn, M. and Weber, K., 1982. Cell 31, 303.
      Application referencesImmunoblotting, Immunocytochemistry, Frozen Sections, Paraffin Sections Droese, M., et al. 1984. Acta. Cytol. 28, 368. Osborn, M., et al. 1984. Cold Spring Harbor Lab., 191. Cross-Reactivity Altmannsberger, M., et al. 1985. Am. J. Pathol. 118, 85. Specificity Droese, M., et al. 1984. Acta. Cytol. 28, 368. Osborn, M., et al. 1984. Cold Spring Harbor Lab., 191. Debus, E., et al. 1983. EMBO J. 2, 2305. Osborn, M. and Weber, K. 1983. Lab. Invest. 48, 372. Altmannsberger, M., et al. 1982. Lab. Invest. 46, 520.