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MABE666 Anti-Heterochromatin Protein-1 Antibody, clone 4C12.1

Anti-Heterochromatin Protein-1 , clone 4C12.1, Cat. No. MABE666, is a highly specific mouse monoclonal antibody that targets Heterochromatin Protein-1 alpha and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: CBX5, HP1A UniProt Number: P45973
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MABE666
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE666
      ReplacesMAB3584
      DescriptionAnti-Heterochromatin Protein-1 Antibody, clone 4C12.1
      Alternate Names
      • Chromobox protein homolog 5
      • Antigen p25
      • HP1 alpha
      Background InformationChromobox protein homolog 5 (UniProt: P45973; also known as Antigen p25, Heterochromatin protein 1 homolog alpha, HP1 alpha) is encoded by the CBX5 (also known as HP1A) gene (Gene ID: 23468) in human. Chromobox protein homolog 5 is a component of centromeric and pericentromeric heterochromatin that associates with chromosomes during mitosis. Chromobox protein homolog 5 associates specifically with chromatin during metaphase and anaphase. It recognizes and binds histone H3 tails methylated at Lys9 (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when Tyr41 of histone H3 is phosphorylated. It can interact with lamin-B receptor (LBR) and this interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. It is also involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Heterochromatin Protein-1 , clone 4C12.1, Cat. No. MABE666, is a highly specific mouse monoclonal antibody that targets Heterochromatin Protein-1 alpha and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Heterochromatin Protein-1 in human tonsil, human pancreas, and human uterus tissues.
      Biological Information
      ImmunogenGST/His-tagged recombinant fraction corresponding to 68 amino acids from the internal region of human Heterochromatin protein 1 homolog alpha.
      Clone4C12.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CBX5
      • HP1A
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~23 kDa observed; 22.22 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH/3T3 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Heterochromatin Protein-1 in 10 µg of NIH/3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE666 04054839201325