14-173 MAP Kinase 2/Erk2 Protein, active, mouse, 10 µg

10 µg  Also available in 250 µg size --call for pricing and availability and reference catalog number 14-173M when ordering the 250 µg size.
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service


      Contact Customer Service

      Click To Print This Page


      Replacement Information
      Catalogue Number14-173
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionMAP Kinase 2/Erk2 Protein, active, mouse, 10 µg
      OverviewN-terminal GST-tagged, recombinant MAP Kinase 2, expressed in E. coli cells; activated using MEK1 (Catalog #14-429).
      Product Information
      Presentation50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 50% glycerol, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
      ApplicationActive, N-terminal GST-tagged, recombinant MAP Kinase 2, expressed in E. coli cells, activated using MEK1 (Catalog #14-429). For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      Sourcerecombinant, mouse, full-length MAP Kinase 2
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
      Gene Symbol
      • MAPK1
      • p40
      • PRKM2
      • MAPK2
      • PRKM1
      • p42-MAPK
      • p38
      • p41
      • ERK
      • ERK-2
      • ERT1
      • P42MAPK
      • p41mapk
      • ERK2
      Protein TargetMAPK2
      Purification MethodGlutathione agarose; Ni2+/NTA agarose
      Target Sub-FamilyCMGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P63085 #Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The the MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis.
      COFACTOR: Magnesium (By similarity).
      DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
      SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
      Molecular Weight67.8kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by phosphorylation myelin basic protein
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore in undiluted aliquots at -20°C for up to one year from the date of receipt. Avoid repeatede freeze-thaw cycles.
      Packaging Information
      Material Size10 µg
      Material PackageAlso available in 250 µg size --call for pricing and availability and reference catalog number 14-173M when ordering the 250 µg size.
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MAP Kinase 2, active - D8KN012U-CD8KN012U-C
      MAP Kinase 2, active - D8KN012U-ED8KN012U-E
      MAP Kinase 2, active - D8KN012U-FD8KN012U-F


      Reference overviewApplicationPub Med ID
      Ataxia Telangiectasia Mutated Proteins, MAPKs, and RSK2 Are Involved in the Phosphorylation of STAT3
      Zhang, Y., et al
      J Biol Chem, 278:12650-9 (2003)  2003

      Kinase Assay12562765 12562765
      alpha7 Nicotinic Acetylcholine Receptors Mediate {beta}-Amyloid Peptide-induced Tau Protein Phosphorylation
      Wang, H. Y., et al
      J Biol Chem, 278:31547-31553 (2003)  2003

      Kinase Assay12801934 12801934
      Signal transduction pathways involved in phosphorylation and activation of p70S6K following exposure to UVA irradiation
      Zhang, Y., et al
      J Biol Chem, 276:20913-23 (2001)  2001

      Kinase Assay11279232 11279232
      ERKs and p38 kinases mediate ultraviolet B-induced phosphorylation of histone H3 at serine 10.
      Zhong, S P, et al.
      J. Biol. Chem., 275: 20980-4 (2000)  2000

      Show Abstract
      Immunoblotting (Western)10806218 10806218
      Specificity and mechanism of action of some commonly used protein kinase inhibitors.
      Davies, S P, et al.
      Biochem. J., 351: 95-105 (2000)  2000

      Show Abstract
      Kinase Assay10998351 10998351
      Mitogen-activated protein kinase phosphorylates and regulates the HIV-1 Vif protein
      Yang, X. and Gabuzda, D.
      J Biol Chem, 273:29879-87 (1998)  1998

      Activation Assay9792705 9792705
      Mitogen-activated protein (MAP) kinase regulates production of tumor necrosis factor-alpha and release of arachidonic acid in mast cells. Indications of communication between p38 and p42 MAP kinases
      Zhang, C., et al
      J Biol Chem, 272:13397-402 (1997)  1997

      Immunoblotting (Western)9148963 9148963
      Contractile activity stimulates the c-Jun NH2-terminal kinase pathway in rat skeletal muscle
      Aronson, D., et al
      J Biol Chem, 272:25636-40 (1997)  1997

      Kinase Assay9325285 9325285
      Insulin- and mitogen-activated protein kinase-mediated phosphorylation and activation of peroxisome proliferator-activated receptor gamma
      Zhang, B., et al
      J Biol Chem, 271:31771-4 (1996)  1996

      Kinase Assay8943212 8943212
      Identification of insulin-stimulated protein kinase-1 as the rabbit equivalent of rskmo-2. Identification of two threonines phosphorylated during activation by mitogen-activated protein kinase.
      Sutherland, C, et al.
      Eur. J. Biochem., 212: 581-8 (1993)  1993

      Show Abstract
      8444194 8444194

      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      14-173M MAP Kinase 2/Erk2 Protein, active, mouse, 250 µg Show Pricing & Availability


      Catalogue Number Description  
      20-101 Protein Dilution Buffer A Show Pricing & Availability

      Product Families


      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases
      Life Science Research > Proteins and Enzymes > Purified Kinases