14-198 | MAP Kinase 2/Erk2 Protein, unactive, Mouse, 50 µg

14-198
50 µg  
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      Overview

      Replacement Information
      Description
      Catalogue Number14-198
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionMAP Kinase 2/Erk2 Protein, unactive, Mouse, 50 µg
      OverviewRecombinant full-length mouse p42 MAP Kinase 2/Erk2 fused with GST at the N terminus
      References
      Product Information
      Applications
      ApplicationUnactive, recombinant full-length mouse p42 MAP Kinase 2/Erk2 fused with GST at the N terminus, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      Sourceexpressed in E coli
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
      Gene Symbol
      • MAPK1
      • p40
      • PRKM2
      • MAPK2
      • PRKM1
      • p42-MAPK
      • p38
      • p41
      • ERK
      • ERK-2
      • ERT1
      • P42MAPK
      • p41mapk
      • ERK2
      Protein TargetMAPK2
      Purification MethodGlutathione-agarose
      Target Sub-FamilyCMGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P63085 #Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The the MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis.
      COFACTOR: Magnesium (By similarity).
      DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
      SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
      Molecular Weight67.8 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of MBP (Catalog # 13-104) substrate in vitro.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MAP Kinase 2/Erk2, unactive - 1684881-A 1684881-A
      MAP Kinase 2/Erk2, unactive - 1684881-E 1684881-E
      MAP Kinase 2/Erk2, unactive - 1684881-F 1684881-F

      References

      Reference overviewApplicationPub Med ID
      Paclitaxel induces prolonged activation of the Ras/MEK/ERK pathway independently of activating the programmed cell death machinery.
      Okano J, and Rustgi, A K
      J. Biol. Chem., 276: 19555-64 (2001)  2001

      Show Abstract
      Kinase Assay11278851 11278851
      Raf-MEK-Erk cascade in anoikis is controlled by Rac1 and Cdc42 via Akt.
      Zugasti, O, et al.
      Mol. Cell. Biol., 21: 6706-17 (2001)  2001

      Show Abstract
      Immunoprecipitation11533257 11533257
      Contractile activity stimulates the c-Jun NH2-terminal kinase pathway in rat skeletal muscle
      Aronson, D., et al
      J Biol Chem, 272:25636-40 (1997)  1997

      Kinase Assay9325285 9325285

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      Product Families

      Categories

      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases
      Life Science Research > Proteins and Enzymes > Purified Kinases