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NS1226-1MG Cysteine Sulfenic Acid Probe (DCP-Bio1)

Cysteine Sulfenic Acid Probe (DCP-Bio1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
NS1226-1MG
1 mg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberNS1226-1MG
      DescriptionCysteine Sulfenic Acid Probe (DCP-Bio1)
      Background InformationCysteine residues in certain proteins can be oxidized transiently to cysteine sulfenic acid, and such oxidation events may affect enzymatic activity and thus regulate signaling pathways. Most proteins containing cysteine sulfenic acid only do so when the cell contains elevated levels of reactive oxygen species although some proteins, such as GAPDH, appear to contain cysteine sulfenic acid under ordinary conditions.
      The most commonly used method for assessing whether proteins are modified to contain cysteine sulfenic acid employs derivitization with 5,5-dimethyl-1,3-cyclohexanedione (dimedone), and subsequent detection with an antibody against dimedone-modified proteins. However, new reagents have been developed to simplify the analysis and isolation of proteins bearing cysteine sulfenic acid. DCP-Bio1, which consists of the sulfenic acid-reactive 3-(2,4-dioxocyclohexyl) propyl (DCP) appended to biotin, is a useful reagent for characterizing cellular cysteine sulfenic acid.
      References
      Product Information
      Components
      • DCP-Bio1 (Part No. CS211419): 1 mg dry powder, stored under argon.
      Formulation
      Quality LevelMQ100
      Applications
      Application NotesPROTOCOL
      Solutions:
      DCP-Bio1 stock solution
      Resuspend entire contents of vial in 10 µL DMSO for a final concentration of 250 mM
      Stock lysis buffer
      50 mM Tris-HCl, pH 7.5
      100 mM Sodium Chloride
      0.1% SDS
      0.5% Sodium Deoxycholate
      0.5% NP-40
      0.5% Triton X-100
      Working lysis buffer
      On the day of the assay, supplement stock lysis buffer with:
      50 mM Sodium Fluoride
      1 mM PMSF
      1 mM DCP-Bio1
      100 µM DTPA (Diethylene triamine pentaacetic acid)
      10 mM N-ethylmaleimide
      10 mM iodoacetamide
      200 U/mL catalase
      1x protease inhibitor cocktail
      Cell treatment and lysis:
      1) Seed adherent cells in 100 mm cell culture dishes, or other plate as desired.
      2) Change to fresh growth media 30 minutes before selected treatments.
      3) Wash all plates 2 times with 10 mL 1x PBS.
      4) Add 150 µL working lysis buffer to plates and scrape cells.
      5) Incubate lysates on ice for 1-2 hours.
      6) Centrifuge lysates at 10,000 rpm for 10 minutes and collect supernatant.
      7) Remove un-reacted DCP-Bio1 and other small molecules using Bio-Rad P6-Spin Columns (PN: 732-6227) following manufacturer’s instructions.
      8) Measure protein concentration.
      9) Perform biochemical analysis of interest.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe product can be stored at -20ºC. The dry powder, if unopened and stored properly, is usable for at least 4 months from date of receipt. Once resuspended the DCP-Bio-1 solution should be stored in the dark at -20°C.
      TOXICITY: Although the compound appears to be of low toxicity, it has not been extensively evaluated and therefore routine laboratory caution should be exercised.
      Not intended for human consumption.
      Important Note: During shipment, small amounts of product will occasionally become entrapped in the seal of the product vial. For products with masses of 5 mg or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any powder in the container’s cap.
      Packaging Information
      Material Size1 mg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      NS1226-1MG 04053252824784

      Documentation

      Cysteine Sulfenic Acid Probe (DCP-Bio1) SDS

      Title

      Safety Data Sheet (SDS) 

      Cysteine Sulfenic Acid Probe (DCP-Bio1) Certificates of Analysis

      TitleLot Number
      Cysteine Sulfenic Acid Probe DCP-Bio1 - 2287788 2287788
      Cysteine Sulfenic Acid Probe DCP-Bio1 - Q2145580 Q2145580
      Cysteine Sulfenic Acid Probe -2747989 2747989
      Cysteine Sulfenic Acid Probe DCP-Bio1 3108759
      Cysteine Sulfenic Acid Probe DCP-Bio1 2930993
      Cysteine Sulfenic Acid Probe DCP-Bio1 2981027
      Cysteine Sulfenic Acid Probe DCP-Bio1 2482277
      Cysteine Sulfenic Acid Probe DCP-Bio1 2834519
      Cysteine Sulfenic Acid Probe DCP-Bio1 2831337
      Cysteine Sulfenic Acid Probe DCP-Bio1 - 3249940 3249940
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