|DNA Template Prep and Sequencing Reaction Cleanup|
|MultiScreen 384-PCR Filter Plate: Automation-friendly 384-well plate for high-throughput purification of PCR* products|
|PCR Amplification on a Microarray of Gel-Immobilized Oligonucleotides: Detection of Bacterial Toxin- and Drug-Resistant Genes and Their Mutations|
Strizhkov, B.N. Drobyshev, A.L. Mikhailovich, V.M. Mirzabekov, A.D.
BioTechniques 29:844-857 2000
|A Concise Guide to cDNA Microarray Analysis|
P. Hegde, R. Qi, K. Abernathy, C. Gay, S. Dharap, R. Gaspard, J.E. Hughes, E. Snesrud, N. Lee, and J. Quackenbush
BioTechniques Vol. 29, No. 3: pp 548-562 (Sep 2000) 2000
|A specific and sensitive PCR assay suitable for large-scale detection of toxigenic Pasteurella multocida in nasal and tonsillar swabs specimens of pigs|
Kamp, E.M. etal., J.Vet Diagn Invest 8:, 304-309
J.Vet Diagn Invest 8:, 304-309 1996
|Nucleic Acid Purification and Concentration|
|Use of MultiScreen Plates for the Preparation of Bacterial DNA Suitable for PCR|
Reek, F.H., Smits, M.A., Kamp, E. M., Smith, H.E.
BioTechniques. 19: 2, 282 – 285 1995
|Nucleic Acid Purification and Concentration|
|Can the Montage PCR-96 Kit be used in a procedure employing centrifugal rather than vacuum filtration?||Use of the Montage PCR-96 Kit in a centrifugal protocol is not recommended.|
|Is a wash step required following the initial filtration with the Montage PCR-96 Cleanup Kit?||For many applications, a wash step is not necessary. If you need wash the purified PCR products, use the nuclease free water included in the PCR cleanup kit.|
|Is the Montage PCR-96 kit capable of eliminating Triton or other detergents from the buffers used in PCR reactions?||It is difficult to completely remove Triton. We recommend the use of a PCR buffer that is Triton-free. Alternatively, one can use MultiScreen FB plates with TN063 protocol.|
|Can the MultiScreen-PCR plate be used in a centrifugal mode?||The MultiScreen-PCR plates are designed for use in the vacuum mode and use in the centrifugal mode is not recommended.|
|My recoveries with the MultiScreen384-PCR plate are lower than expected. How can I improve them?||Here a few things to check:
|What is the minimum recovery volume possible when using the MultiScreen-PCR plates?||The minimum recovery volume is 50µl. Use of smaller volumes can be used but will result in a decrease in DNA recovery.|
|What are the dimensions of the Multiscreen-PCR plate?||The plate length is 123.4mm. The plate width is 82.7mm. the plate depth is 14.6mm without the cover. 16.5mm with cover.|
|When using the Montage kits how can I tell when filtration in the MultiScreen plate is complete?||Filtration is complete when the individual wells of the MultiScreen plate are empty and the membranes appears shiny. Filtration usually takes about 10 minutes, depending on starting volume.|
|What is the maximum vacuum that can be applied to the Montage PCRu96 plate?||The recommended vacuum pressure for PCR purification is 20 inches of Hg. Exceeding this vacuum pressure will adversely affect recovery of PCR products and is not recommended.|
|How do I resuspend the samples from the Montage PCRm96 plate?||Maximal recovery of purified PCR products requires efficient dissolution of the concentrated nucleic acid from the membrane surface. There are several efficient methods that can be used for resuspension of PCR products form the PCRm96 plate. The choice of a resuspension method should take into account the equipment available, automation and the desired throughput and/or processing time.
Incubate the plate at room temperature for 20 minutes followed by pipetting up and down once. Transfer the samples to a suitable collection plate.
1. Shaking the plate for 10 minutes on a plate shaker (e.g., Lab-Line Titer Plate Shaker Model 4625 set at 1100 rpm). Transfer to collection plate.
2. Shake the plate for 10 minutes on at setting #5 on vortex mixer (fitted with a microwell plate adapter) for 10 minutes. Transfer to collection plate.
Resuspension via an automated liquid handler
The Beckman Multimek-96 workstation can be used to dissolve the samples in 20 mL by adjusting the tip height to above the membrane surface and mixing 15 mL for 25-50 cycles at 50% pipetting speed.
Note: Other pipetting workstations may also be used to efficiently resuspend purified products from the Montage PCRm96 plate. However, optimization of the resuspension conditions for any particular liquid handlers is required.
|Montage PCR<sub>96</sub> Clean-Up Kit Laminated Card|
|Montage PCR<sub>96</sub> Clean-Up Kit User Guide|
|MultiScreen 384 PCR Plate|
|MultiScreen PCR µ96 Plate|