PCR Cleanup Filter Plates

Remove primers and unincorporated dNTPs in one step

Overview

Specifications

Ordering Information

Filter Plates Clear Sorting & Filtering
Catalogue Numbericon Descriptionicon Pack Sizeicon
LSKMPCR10MultiScreen PCRµ96 Filter Plate 10 Show Pricing & Availability
LSKMPCR50MultiScreen-PCRµ96 Filter Plate 50 Show Pricing & Availability
MSNU03010MultiScreen-PCR96 Filter Plate 10 Show Pricing & Availability
MSNU03050MultiScreen-PCR96 Filter Plate 50 Show Pricing & Availability
S384PCR10MultiScreen PCR384 Filter Plate 10 Show Pricing & Availability
S384PCR50MultiScreen PCR384 Filter Plate 50 Show Pricing & Availability

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Required Equiment Clear Sorting & Filtering
Catalogue Numbericon Descriptionicon Pack Sizeicon
MSVMHTS00MultiScreenHTS Vacuum Manifold 1 Show Pricing & Availability
WP6110060Chemical Duty Pump, 100 V/50–60 Hz 1 Show Pricing & Availability
WP6111560Chemical Duty Pump, 115 V/60 Hz 1 Show Pricing & Availability
WP6122050Chemical Duty Pump, 220 V/50 Hz 1 Show Pricing & Availability

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    Documentation

    Technical Info

    Title
    PCR Purification Using Montage PCR96 Cleanup Kits on the Packard MultiPROBE® II EX Liquid Handling System
    PCR Purification Using Montage PCR96 Cleanup Kits on the Tecan Genesis® RSP
    PCR Purification Using Montage PCR<sub>u96 </sub>Plates on the PerkinElmer MultiPROBE® II HT EX liquid handling system.
    PCR Purification Using Montage PCRu96 Plates on the Tecan MiniPrep 60 MolBio Instrument
    PCR Purification Using the Montage PCR96 Cleanup Kit on the Qiagen BioRobot 3000 Workstation.
    PCR Purification Using the Montage PCR96 Cleanup Kit on the Tecan MiniPrep 60 MolBio Instrument

    Data Sheet

    Title
    DNA Template Prep and Sequencing Reaction Cleanup
    MultiScreen 384-PCR Filter Plate: Automation-friendly 384-well plate for high-throughput purification of PCR* products

    Posters

    Title
    High Density Sample Preparation Prior To Capillary Electrophoresis Sequencing; 2000
    Increasing Throughput and Quality of Sequencing by Automation
    Poster: An Industrialized Platform for Yeast Two-Hybrid Screening
    Poster: Montage PCRµ96 and SEQ96: A Total Solution for Sequencing PCR-derived Templates
    Poster: Purification Automation of Small Volume PCR Purification

    References

    Reference overviewApplication
    PCR Amplification on a Microarray of Gel-Immobilized Oligonucleotides: Detection of Bacterial Toxin- and Drug-Resistant Genes and Their Mutations
    Strizhkov, B.N. Drobyshev, A.L. Mikhailovich, V.M. Mirzabekov, A.D.
    BioTechniques 29:844-857  2000

    A Concise Guide to cDNA Microarray Analysis
    P. Hegde, R. Qi, K. Abernathy, C. Gay, S. Dharap, R. Gaspard, J.E. Hughes, E. Snesrud, N. Lee, and J. Quackenbush
    BioTechniques Vol. 29, No. 3: pp 548-562 (Sep 2000)  2000

    PCR
    A specific and sensitive PCR assay suitable for large-scale detection of toxigenic Pasteurella multocida in nasal and tonsillar swabs specimens of pigs
    Kamp, E.M. etal., J.Vet Diagn Invest 8:, 304-309
    J.Vet Diagn Invest 8:, 304-309  1996

    Nucleic Acid Purification and Concentration
    Use of MultiScreen Plates for the Preparation of Bacterial DNA Suitable for PCR
    Reek, F.H., Smits, M.A., Kamp, E. M., Smith, H.E.
    BioTechniques. 19: 2, 282 – 285  1995

    Nucleic Acid Purification and Concentration

    FAQ

    QuestionAnswer
    Can the MultiScreen-PCR plate be used with another manufacturers vacuum manifold?No, the plate needs the support grid of the Millipore vacuum manifold.
    I overlay mineral oil on my PCR reactions before I run them in the thermocycler. Will the mineral oil pose a problem when I perform the dye terminator clean-up procedure with the MultiScreen plates?The mineral oil will not interfere with the dye terminator clean-up. We have run PCR reactions through a G-50 loaded MultiScreen plate and found no reduction in signal strength or sequencing accuracy of the purified product.
    Will mineral oil be removed by the MultiScreen 384-PCR plate?No. Oil will resuspend with PCR products. All 384 thermocyclers use "Hot Bonnet" technology, however, eliminating the need for oil.
    When using the Multiscreen PCR plates, what is the minimum and maximum DNA loading capacity per well?The minimum DNA loading capacity is one microgram per well. There is no upper limit.
    When using the Montage PCR-96 kit, what is the recommended procedure for resuspension of PCR fragments post filtration?Avoid manual pipetting. The use of a plate shaker or automated pipettor (on robotic liquid handling system) is highly recommended.
    I am using the Multiscreen PCR plate and losing 20-50 percent of my final resuspended sample after agitation. How can I correct for this?1) After the initial vacuum filtration of your sample be sure to blot the bottom of the plate well on a paper towel. Do not put the plate on anything with wicking capability after that.
    2) Readjust the agitation speed of your plate shaker following the guidelines in the Multiscreen-PCR plate operating manual.
    Can the Montage PCRm96 plate be used in centrifugal mode?No. The Montage PCRm96 plate is designed for purification using vacuum filtration only.
    If I dilute my sample when using the Montage PCRu96 plate, will that increase the amount of time for filtration to be completed?Yes. Although sample dilution will marginally increase processing time, dilution is essential for maximal recovery of PCR products.
    Is a wash step required following the initial filtration when using the Montage PCRu96 plate?The PCR product is sufficiently pure for DNA sequencing after a single vacuum filtration. If a particular application demands even higher purity, a wash step may be added.
    Is the Montage PCRm96 plate capable of removing Triton or other detergents from the PCR reaction?No. The use of PCR reaction buffers that contain high concentrations of surfactants (i.e., greater than the critical micelle concentration) or protein stabilizers (e.g., gelatin) is not recommended for this application. Surfactants including Tween-20, Triton X-100 and Nonidet P-40 are not efficiently removed by the Montage PCRm96 plates and may result in carry-over into subsequent reactions.

    User Guides

    Title
    Montage PCR<sub>96</sub> Clean-Up Kit Laminated Card
    Montage PCR<sub>96</sub> Clean-Up Kit User Guide
    MultiScreen 384 PCR Plate
    MultiScreen PCR µ96 Plate