|Clarification and Recovery of Recombinant Proteins using a Cascade Tangential Flow Filtration (TFF) System|
|What is Normalized Water Permeability?||NWP is a measure of the ability of pure water to pass through the filter membrane. NWP is a property of the TFF system and is contributed to by the membrane as well as the feed and permeate flow paths in the filter.|
|How does NWP aid in effective cleaning procedure development?||Fouling and polarization leave residues on the membrane that can reduce filter permeability. Effective cleaning removes these residues, restoring the permeability of the membrane. Ideally, NWP after cleaning should equal NWP before use although there can be variation up to +/-15% due to membrane compaction under pressure and measurement error.
NWP validates the ability of cleaning to restore filtration rate of a system.
|How is NWP measured?||NWP is measured by filtering pure water throught he system at controlled cross flow, Trans Membrane Pressure and temperature. The filtrate flow rate is measured. The flow rate is normalized to the membrane area, TMP and temperature.
Please refer to the operator's manual or contact Techical Service for further questions regarding the calculation of NWP.
Custom Engineered SystemsFor chromatography systems, tangential flow filtration systems, integrated systems.
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|Biopharmaceutical Manufacturing > Upstream Processing > Cell Culture > Cell Culture Clarification > Millistak+ Pod > Prostak|
The Prostak Open-Channel module is the membrane-based separations device in Prostak Tangential Flow Filter (TFF) Systems. The module itself is a tangential flow stacked plate device with an open feed channel. The modules are solvent-bonded and contain neither adhesives nor compression-dependent membrane seals. Prostak open-channel modules using Durapore microporous membranes are steam sterilizable for at least 20 cycles at 126 °C for 1 hour at less than 0.14 bar (2 psi) feed to retentate pressure.
- Mammalian, bacterial and mycelial cell suspensions
- Emulsions and colloidal suspension
- Viruses, proteins and other bio-organic macromolecular solutions
- Polysaccharides and other high viscosity solutions
- Yeast, algae, and other high solids suspensions
- Protein precipitates
|Weight||1.6 kg (3.5 lb.)||2.1 kg (4.7 lb.)||3.6 kg (7.9 lb.)|
|Length||38.9 cm (15.3 in.)||38.9 cm (15.3 in.)||38.9 cm (15.3 in.)|
|Width||19.8 cm (7.8 in.)||19.8 cm (7.8 in.)||19.8 cm (7.8 in.)|
|Height||4.6 cm (1.8 in.)||5.6 cm (2.2 in.)||8.1 cm (3.2 in.)|
|Internal Volume Total, mL||500||700||1250|
|Feed Side, mL||300||420||750|
|Filtrate Side, mL||200||280||500|
|Filtration Area||0.17 m²||0.33 m²||0.84 m²|
|Materials of Construction|
|Membrane||Hydrophilic PVDF or Hydrophobic PVDF|
|Plate||Polysulfone, 10% glass filled|
|Gaskets||Filters provided with silicone gaskets, EPDM gaskets also available|
|Operating Guidelines||Process and
|Maximum Inlet Pressure|
|at < 50 °C (120 °F)||5.6 bar (80 psi)*|
|at 50–80 °C (120–180 °F)||4.2 bar (60 psi)|
|at 100–132 °C (212–270 °F)||2.7 bar (40 psi)|
|Maximum Temperature||90 °C (195 °F)||135 °C (275 °F)|
|Maximum Transmembrane Pressure - Forward|
|at < 50 °C (120 °F)||4.2 bar (60 psi)*|
|50–80 °C (120–180 °F)||2.1 bar (30 psi)|
|100–132 °C (212–270 °F)||0.14 bar (2 psi)|
|pH Range - short duration (1 hr cycle)||1–11|
|pH Range - continuous||2–10|
|Prefiltration Required||100 µm|
|* Durapore DV membrane — 3.5 bar (50 psi) maximum pressure forward|