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S7842 CpG WIZ® H19-IGF2 Amplification Kit

The components of the CpG WIZ H19-IGF2 Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples.

CpG WIZ® H19-IGF2 Amplification Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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S7842
25 assays  
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      Overview

      Replacement Information
      Description
      Catalogue NumberS7842
      Brand Family Chemicon®
      Trade Name
      • CpG Wiz
      • Chemicon
      DescriptionCpG WIZ® H19-IGF2 Amplification Kit
      OverviewMethylation of cytosines located 5' to guanosine is known to have a profound effect on the expression of several eukaryotic genes (Bird, 1992 ). In normal cells, methylation occurs predominantly in CG-poor regions, while CG-rich areas, called CpG islands, remain unmethylated. Aberrant methylation of normally unmethylated CpG islands has been documented as a relatively frequent event in immortalized and transformed cells (Antequera, 1990) and has been associated with transcriptional inactivation of defined tumor suppressor genes in human cancers (Merlo, 1995). Aberrant methylation of H19-IGF2 locus is implicated in several inherited genetic disorders.



      Methylation-specific PCR (MSP) is a new technology for sensitive detection of abnormal gene methylation utilizing small amounts of DNA (Herman, 1996 ). This process employs an initial bisulfite reaction to modify the DNA, followed by PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA.
      Materials Required but Not Delivereda. Microcentrifuge tubes for PCR amplification
      b. Aerosol-resistant pipette tips
      c. Thermocycler
      d. Gel electrophoresis apparatus (vertical or horizontal)
      e. Power Supply
      f. 302 nm UV transilluminator, camera and film
      Reagents
      a. 2.5 mM dNTP mix (2.5 mM of each nucleotide)
      b. "Hot start" Taq polymerase
      c. "Hot start" PCR reagents (see Sec. II. Protocols).
      d. Reagents for gel electrophoresis (1X TBE and 2% agarose, 10% acrylamide, or suitable high resolution agarose)
      e. DNA markers (size range 100-300 bp)
      f. Ethidium bromide (10 mg/mL)
      g. Gel-loading solution / Loading Dye
      h. . Bisulfite Modified DNA (CpGenome™ DNA Modification Kit, Cat. No. S7820; CpGENOME™ Fast DNA Modification Kit, Cat. No. S7824)
      References
      Product Information
      Components
      • The components of the CpG WIZ® H19-IGF2 Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples with appropriate controls.
      • U Primer Set, part # 90669
      • M Primer Set, part #90668
      • W Primer Set, part #90670
      • Normal Control DNA part #90377
      • Universal 10X PCR Buffer, part #90396
      Quality LevelMQ100
      Applications
      ApplicationThe components of the CpG WIZ H19-IGF2 Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples.
      Key Applications
      • PCR
      Application NotesPrinciples of the Technique

      Use of either the CpGenome™ DNA Modification Kit (Cat. No. S7820) or the CpGENOME™ Fast DNA Modification Kit (Cat. No. S7824) facilitates the initial bisulfite reactions, while the CpG WIZ® H19-IGF2 Amplification Kit contains the reagents required for the gene-specific PCR amplification reactions.
      Biological Information
      Species Reactivity
      • Human
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is an imprinted gene and is expressed only from the paternally inherited allele. It is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3' region and to the upstream INS gene at the 5' region. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.
      Gene Symbol
      • IGF2
      • ORFNames=PP1446
      • INSIGF
      • FLJ22066
      • FLJ44734
      • pp9974
      • C11orf43
      • IGF-II
      • Somatomedin-A
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01344 # The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
      SIZE: 180 amino acids; 20140 Da
      SUBCELLULAR LOCATION: Secreted.
      SIMILARITY: SwissProt: P01344 ## Belongs to the insulin family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size25 assays
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      S7842 04053252665752