03-230 | SIRT5 (His-tag) human recombinant - HDAC protein

03-230
500 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Key ApplicationsSpecies
      Enzyme AssaysHuman
      Description
      Catalogue Number03-230
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionSIRT5 (His-tag) human recombinant - HDAC protein
      OverviewHuman recombinant SIRT5 (His-tag) expressed in E. Coli
      Background InformationSIRT5, along with SIRT 3 and 4, is one of three human mitochondrial sirtuins (homologs of yeast Sir2). SIRT5, which is localized to both the mitochondrial matrix and the intermembrane space, is an NAD+-dependent lysine deacetylase (class III HDAC). SIRT5’s only known physiological target is the intermembrane space protein, cytochrome c, suggesting the possibility of regulatory roles in metabolism and/or apoptosis. SIRT5 has also been shown to deacetylate and thereby upregulate the matrix urea cycle enzyme carbamoyl phosphate synthetase 1. This implies an important role for SIRT5 in ammonia detoxification and adaptation to elevated amino acid catabolism during starvation, calorie restriction, or a high protein diet. SIRT5 is most strongly expressed in thymus, lymphoblasts, and heart muscle cells and its chromosomal location (6p23) has been implicated in chromosomal abnormalities associated with malignancies.
      References
      Product Information
      FormatPurified
      PresentationIn liquid containing 25 mM Tris, pH 7.5, 100 mM NaCl, 5 mM DTT, containing 10% glycerol. Frozen solution.
      Applications
      ApplicationHuman recombinant SIRT5 (His-tag) expressed in E. Coli.
      Key Applications
      • Enzyme Assays
      Application NotesHDAC deacetylase assays
      Biological Information
      HostE. coli
      PurityPurity >95% by SDS-PAGE
      SourceRecombinant residues 37-310 of human SIRT5 isoform 1, with an N-terminal His-tag
      Specific Activity>200 pmol/min/mg at 37ºC, as determined by a fluorometric activity assay.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -80°C. The enzyme is stable on ice for 30 to 60 min, but may lose activity with dilution and/or prolonged storage on ice. It is recommended that thawing and addition of the enzyme to assay wells be done within as short a time as possible before start of the assay and that the thawed enzyme be kept on ice throughout. Best performance will be obtained by adding the chilled, undiluted enzyme directly to pre-warmed buffer and proceeding immediately to the addition of pre-warmed substrate. The remaining, unused enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the SIRT5 into separate tubes before storing at -80°C.
      Packaging Information
      Material Size500 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Proteins and Enzymes > Other Enzymes