Water for Spectophotometry

 
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Application Overview


Water for UV-Vis Spectrophotometry

Many molecules absorb in the ultraviolet (UV) and visible (Vis) range of the electromagnetic spectrum. UV radiation covers the range from 190-350 nm, while visible radiation covers the 350-800 nm range. The absorption of radiation corresponds to the excitation of outer electrons in the molecule. In a spectrophotometer, radiation with a specific intensity is passed through a liquid sample that is contained in a cuvette (usually made of quartz). If the sample contains species that absorb(s) in that specific radiation wavelength, the radiation intensity emerging on the other side of the cuvette is reduced. This phenomenon is used to identify and/or quantify a molecule in a sample. Compounds have unique UV-Vis spectra, including a maximum absorption wavelength (λmax) and molar extinction coefficient (ε), so UV-Vis can be used to identify the presence of chemicals in samples. Because of this, UV-Vis is a popular technique in chemistry, foods, pigments, pharmaceuticals, polymers, and the life sciences, for basic or applied research as well as quality control.

Quantitation in UV-Vis spectrophotometry is expressed by Beer-Lambert’s Law:

Aλ = ελ c l

where = absorbance at wavelength λ, ελ = extinction coefficient at wavelength λ, c = concentration, and l = pathlength. In most experiments, ε and l are constant, therefore absorbance is proportional to the concentration of the compound.

UV-Vis spectrophotometers can be either single beam or double beam in design. In single-beam instruments, all the light passes through the sample cell, therefore a correction for the loss of light intensity as the beam passes through the solvent must be made. This is done by replacing the sample by a blank or reference sample to account for any matrix effects. Dual-beam spectrophotometers split the light into two beams before it reaches the sample. One beam is used as a reference beam, and the other passes through the sample. Correction is performed automatically.


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Events

Jul 09
09/07/15
10th Chemistry and Biology of Peptides Meeting
Francis Crick Institute
London, England
Water Purification,Reagents Chemicals and Labware,Industrial Biomonitoring,IVD/OEM Materials and Reagents,Analytics and Sample Preparation Europe 20150709
Jul 10
10/07/15
Deelname 12e VCCN Nationale Cleanroom Dag
1402 SB, Doctor Abr
Bussum, Netherlands
Water Purification,Reagents Chemicals and Labware,Life Science Research,Industrial Biomonitoring,IVD/OEM Materials and Reagents,Analytics and Sample Preparation Europe 20150710
Jul 25
25/07/15 - 28/07/15
IAFP Annual Meeting
Oregon Convention Center
Portland, OR, USA
Water Purification,Biopharmaceutical Manufacturing,Analytics and Sample Preparation North America 20150725
Jul 26
26/07/15 - 30/07/15
AACC Annual Meeting & Clinical Lab Expo
Georgia World Congress Center
Atlanta, GA, USA
Water Purification,Analytics and Sample Preparation North America 20150726
Jul 29
29/07/15 - 31/07/15
PRChem
The Ritz-Carlton Hotel
San Juan, USA
Reagents Chemicals and Labware,Industrial Biomonitoring,Analytics and Sample Preparation North America 20150729