Pour les questions d'ordre général, veuillez contacter notre Service Clients :
Merck Chemicals Ltd.
Padge Road, Beeston
NG9 2JR Nottingham
United Kingdom
Téléphone : +44 (0)115 9430840
Fax : +44 (0)115 9430951
e-mail : customer.service@merckbio.eu
30 mai 2012
Chargement
This product has been discontinued.
We are offering Glutathione Peroxidase Assay Kit (Cat. No. 353919) as a possible alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.
A spectrophotometric assay kit based on the method described by Paglia and Valentine. Glutathione peroxidase (GPx) catalyzes the reduction of an organic peroxide while forming oxidized glutathione (GSSG). GSSG is reduced to GSH by the enzyme glutathione reductase, and NADPH is oxidized to NADP in the catalytic cycle. GPx activity is quantitated by measuring the change in absorbance at 340 nm caused by the oxidation of NADPH. Each kit can be used to perform up to 100 assays.
| Informations produit | |||
|---|---|---|---|
| Format | Cuvette | ||
| Form | 100 Tests | ||
| Detection method | Colorimetric | ||
| Unit definition | One unit is defined as the amount of enzyme that will cause the oxidation of 1.0 μM of NADPH to NADP per minute at 37°C measured at 340 nm. | ||
| Assay range | 5.6-24 mU/ml | ||
| Assay time | 2 h | ||
| Sample type | Cell lysates, tissue extracts, erythrocyte lysates | ||
| Kit contains | Glutathione, glutathione reductase, NADPH, t-butylhydroperoxide, buffer, and a user protocol. | ||
| Information relative au stockage et expédition | |||
|---|---|---|---|
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Traitement des matériaux dangereux : En raison de la nature dangereuse des matériaux contenus dans cet envoi, des frais d'expédition supplémentaires pourront être appliqués à votre commande. Certaines tailles peuvent être exonérées des frais d'expédition supplémentaires pour matériaux dangereux. Veuillez contacter la filiale la plus proche pour de plus amples informations sur ces frais. |
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| Catégorie de stockage | Multiple Storage Requirements | ||
| Ship |
Multiple Storage Conditions
Multiple Toxicity Values, refer to MSDS |
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| Informations de sécurité | |||
|---|---|---|---|
| Phrase S |
S: 23-36/37/39-16-17 |
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| Phrase R |
R: 8-20/21/22-34-40-44 |
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| Données | |||
|---|---|---|---|
 Cellular Glutathione Peroxidase at a 288 mU/ml assayed at 23°C. |
