Wenn Sie allgemeine Fragen haben, wenden Sie sich bitte an unseren Kundenservice:
Merck Chemicals Ltd.
Padge Road, Beeston
NG9 2JR Nottingham
United Kingdom
Telefon: +44 (0)115 9430840
Fax: +44 (0)115 9430951
E-Mail: customer.service@merckbio.eu
30 Mai 2012
wird geladen
This product has been discontinued.
We are offering Glutathione Peroxidase Assay Kit (Cat. No. 353919) as a possible alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.
A spectrophotometric assay kit based on the method described by Paglia and Valentine. Glutathione peroxidase (GPx) catalyzes the reduction of an organic peroxide while forming oxidized glutathione (GSSG). GSSG is reduced to GSH by the enzyme glutathione reductase, and NADPH is oxidized to NADP in the catalytic cycle. GPx activity is quantitated by measuring the change in absorbance at 340 nm caused by the oxidation of NADPH. Each kit can be used to perform up to 100 assays.
| Produktinformationen | |||
|---|---|---|---|
| Format | Cuvette | ||
| Form | 100 Tests | ||
| Detection method | Colorimetric | ||
| Unit definition | One unit is defined as the amount of enzyme that will cause the oxidation of 1.0 μM of NADPH to NADP per minute at 37°C measured at 340 nm. | ||
| Assay range | 5.6-24 mU/ml | ||
| Assay time | 2 h | ||
| Sample type | Cell lysates, tissue extracts, erythrocyte lysates | ||
| Kit contains | Glutathione, glutathione reductase, NADPH, t-butylhydroperoxide, buffer, and a user protocol. | ||
| Lager- und Versandinformationen | |||
|---|---|---|---|
|
Achtung Gefahrgut:Aufgrund der Natur des in dieser Lieferung enthaltenen Gefahrgutes können zusätzliche Versandkosten für Ihren Auftrag entstehen. Bestimmte Größen können ggf. von den Versandzuschlägen für Gefahrgüter ausgenommen sein. Bitte kontaktieren Sie Ihr lokales Verkaufsbüro für weitere Informationen bzgl. dieser Gebühren. |
|||
| Lagerkategorie | Multiple Storage Requirements | ||
| Ship |
Multiple Storage Conditions
Multiple Toxicity Values, refer to MSDS |
||
| Sicherheitshinweise | |||
|---|---|---|---|
| S-Satz |
S: 23-36/37/39-16-17 |
||
| R-Satz |
R: 8-20/21/22-34-40-44 |
||
| Daten | |||
|---|---|---|---|
 Cellular Glutathione Peroxidase at a 288 mU/ml assayed at 23°C. |
