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Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Telefon: +49 6151 72-0
Faks: +49 6151 72 2000
31 May 2012
memuat
This product has been discontinued.
We are offering Glutathione Peroxidase Assay Kit (Cat. No. 353919) as a possible alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.
A spectrophotometric assay kit based on the method described by Paglia and Valentine. Glutathione peroxidase (GPx) catalyzes the reduction of an organic peroxide while forming oxidized glutathione (GSSG). GSSG is reduced to GSH by the enzyme glutathione reductase, and NADPH is oxidized to NADP in the catalytic cycle. GPx activity is quantitated by measuring the change in absorbance at 340 nm caused by the oxidation of NADPH. Each kit can be used to perform up to 100 assays.
| Informasi produk | |||
|---|---|---|---|
| Format | Cuvette | ||
| Form | 100 Tests | ||
| Detection method | Colorimetric | ||
| Unit definition | One unit is defined as the amount of enzyme that will cause the oxidation of 1.0 μM of NADPH to NADP per minute at 37°C measured at 340 nm. | ||
| Assay range | 5.6-24 mU/ml | ||
| Assay time | 2 h | ||
| Sample type | Cell lysates, tissue extracts, erythrocyte lysates | ||
| Kit contains | Glutathione, glutathione reductase, NADPH, t-butylhydroperoxide, buffer, and a user protocol. | ||
| Simpan dan kirim informasi | |||
|---|---|---|---|
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Informasi Bahan Berbahaya: Karena sifat dari Bahan Berbahaya ini kami mengenakan biaya pengiriman tambahan yang dapat ditambahkan ke pesanan Anda. Kemasan tertentu mungkin saja tidak dikenakan tambahan biaya pengiriman bahan berbahaya. Silakan hubungi kami untuk informasi mengenai biaya tambahan ini. |
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| Penyimpanan | Multiple Storage Requirements | ||
| Ship |
Multiple Storage Conditions
Multiple Toxicity Values, refer to MSDS |
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| Informasi keselamatan kerja | |||
|---|---|---|---|
| Frase S |
S: 23-36/37/39-16-17 |
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| Frase R |
R: 8-20/21/22-34-40-44 |
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| Data | |||
|---|---|---|---|
 Cellular Glutathione Peroxidase at a 288 mU/ml assayed at 23°C. |
