428900
LCAT Activity Assay Kit, Fluorometric
Lecithin:Cholesterol Acyltransferase Activity Assay Kit, Fluorometric
Untuk pertanyaan umum, silakan hubungi Layanan Pelanggan kami:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Telefon: +49 6151 72-0
Faks: +49 6151 72 2000
31 May 2012
memuat
Assay kit useful for the quantitative measurement of lecithin:cholesterol acyltransferase activity (LCAT) in human plasma. LCAT mediates the formation of cholesteryl esters in human plasma by transferring an acyl chain from the sn-2 position of phosphatidylcholine to cholesterol.
| Informasi produk | |||
|---|---|---|---|
| Form | 100 Tests | ||
| Detection method | Fluorometric | ||
| Species reactivity | human | ||
| Protect from Light | Ya | ||
| Assay time | 4.5 - 8.5 h | ||
| Sample type | Plasma | ||
| Kit contains | LCAT Substrate, READ Reagent, and a user protocol. | ||
| Simpan dan kirim informasi | |||
|---|---|---|---|
| Penyimpanan | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Irritant |
||
| Informasi keselamatan kerja | |||
|---|---|---|---|
| Frase S |
S: 26 |
||
| Frase R |
R: 36/38 |
||
| Data | |||
|---|---|---|---|
 The non-hydrolyzed LCAT Substrate fluoresces at 470 nm. Upon hydrolysis by LCAT, a monomer is released from the LCAT Substrate that fluoresces at 390 nm. LCAT activity is assessed as a change in 470/390 emission intensity.  Normal human plasma is assayed according to the Detailed Protocol above. Over time the 470/390 ration decreases, indicating LCAT activity in the sample. |
