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メルク株式会社
目黒区下目黒1-8-1
153-8927 東京
Japan
TEL： 0120-189-390
Fax: 0120-189-350
E-mail mj_service@merckgroup.com
31 5月 2012
ローディング
Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for 7 rare codones (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) on a compatible chloramphenicol-resistant plasmid. The tRNA genes are driven by their native promoters.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability. Genotypes:
Rosetta 2
F- ompT hsdSB(rB- mB-) gal dcm pRARE2 (CamR)
Rosetta 2(DE3)
F- ompT hsdSB(rB- mB-) gal dcm (DE3) pRARE2 (CamR)
Rosetta 2(DE3)pLysS
F- ompT hsdSB(rB- mB-) gal dcm (DE3) pLysSRARE2 (CamR)
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability. Genotypes:
Rosetta 2
F- ompT hsdSB(rB- mB-) gal dcm pRARE2 (CamR)
Rosetta 2(DE3)
F- ompT hsdSB(rB- mB-) gal dcm (DE3) pRARE2 (CamR)
Rosetta 2(DE3)pLysS
F- ompT hsdSB(rB- mB-) gal dcm (DE3) pLysSRARE2 (CamR)
| 関連製品 | |
|---|---|
| 71397 | Rosetta™ 2(DE3) Competent Cells |
| 71402 | Rosetta™ 2 Competent Cells |
| 71403 | Rosetta™ 2(DE3)pLysS Competent Cells |
| 関連製品 for Rosetta™ 2 Competent Cell Set | |
| 71397 | Rosetta™ 2(DE3) Competent Cells |
| 71402 | Rosetta™ 2 Competent Cells |
| 71403 | Rosetta™ 2(DE3)pLysS Competent Cells |
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| Components |
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| 保管と送付情報 | |||
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| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Licence Brookhaven | This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license. | ||
