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Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Telefon: +49 6151 72-0
Fax: +49 6151 72 2000
31 Mai 2012
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The BacMagic™-1 DNA Kit provides a positive selection for creating baculovirus recombinants in preparation for generating recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification. BacMagic DNA is an AcNPV genome with a portion of the essential open reading frame (ORF) 1629 deleted, preventing nonrecombinant virus from replicating in insect cells. The target coding sequence in a compatible transfer plasmid, such as pIEx/Bac™, pBAC™, or pTriEx™ vectors, is cotransfected with BacMagic DNA into insect cells. Homologous recombination within the cells restores the function of the viral ORF1629. Only recombinant baculovirus can replicate, producing a homogeneous population of recombinants. The BacMagic™-1 DNA Kit contains the BacMagic-1 DNA, Insect GeneJuice® Transfection Reagent, and a positive control plasmid to monitor co-transfection efficiency.
Not available for sale in Japan
Not available for sale in Japan
| Produktinformationen | ||||||||||||
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| Components |
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| Aggregation Number | TRANSFECTION CONTROL PLASMID | |||||||||||
| Lager- und Versandinformationen | |||
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| Lagerkategorie | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Daten | |||
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 Figure 1. Construction of baculovirus recombinants with three BacMagic systems  Figure 2: Streamlined baculovirus method |
