For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
31 May 2012
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Recombinant, rat calpain-2 expressed in E. coli. Ca2+-dependent heterodimeric cysteine proteinase with high Ca2+ requirement (EC50 = 350 µM Ca2+). Supplied as highly purified preparation.
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 100 mM NaCl, 50 mM Tris-HCl, 5 mM EDTA, 5 mM β-mercaptoethanol, 40% glycerol, pH 7.8. | ||
| Molar mass | 101000 | ||
| Avoid freeze/thaw | Yes | ||
| Specific activity | ≥1500 units/mg protein | ||
| Unit definition | One unit is defined as the amount of enzyme that hydrolyzes 1 pmol. Suc-leu-Leu0Val-Tyr-AMC in 1 min at 25°C using the Calpain Activity Assay Kit, Fluorometric (Cat. No. QIA120). Note: 1 caseinolytic unit = 0.8 fluorogenic units. | ||
| Purity | ≥98% by SDS-PAGE | ||
| EC number | 3.4.22.17 | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Harmful |
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| Safety information | |||
|---|---|---|---|
| S Phrase |
S: 26-36 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing. |
||
| R Phrase |
R: 36/37/38-42/43 Irritating to eyes, respiratory system and skin. May cause sensitization by inhalation and skin contact. |
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