218828
Caspase-1 Substrate XV, Cell-Permeable, Fluorogenic
Nα-5-Tetramethylrhodaminyl-YVADAC(S-acrylodan)-OH, TMR-YVADAC(AD)
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Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
31 May 2012
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A cell-permeable, internally-quenched fluorogenic peptide substrate that is useful for detecting in vivo caspase-1-like protease activity in living cells or tissues. Efficiently cleaved by caspase-1 in vitro at the peptide bond between Asp and Ala residues (kcat/Km ~ 8.5 x 10-2 µM-1s-1) with an increase in fluorescence intensity (~525 nm) when excited at 380 nm. Both time-dependent and sub-cellular localization of caspase-1-like activity could be visualized in glutamate-induced rat hippocampal cell death.
| Product information | |||
|---|---|---|---|
| Peptide Sequence | TAMRA(5)-Tyr-Val-Ala-Asp-Ala-Cys(S-acrylodan)-OH | ||
| Form | Red-purple lyophilized solid | ||
| Formulation | Supplied as a trifluoroacetate salt. | ||
| Molar mass | 1278.4 | ||
| Protect from Light | Yes | ||
| Protect from Moisture | Yes | ||
| Packaged under inert gas | Yes | ||
| Purity | ≥90% by HPLC | ||
| Solubility | DMSO | ||
| Chemical formula | C67H75N9O15S | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Standard Handling |
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