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Merck KGaA
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Phone: +49 6151 72-0
Fax: +49 6151 72 2000
31 May 2012
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Recombinant, human caspase-10 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 11000 and 18000.
M.W. 11,000 and 18,000 (heterodimer).
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.1% CHAPS, 0.09% APO-10, pH 7.4. | ||
| Avoid freeze/thaw | Yes | ||
| Specific activity | ≥150,000 units/mg protein | ||
| Unit definition | One unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-IETD-pNA (Cat. No. 368057) per min at 25°C, pH 7.4. | ||
| Activity | ≥50,000 units/ml | ||
| Purity | ≥95% by SDS-PAGE | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
||
