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Merck KGaA
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31 May 2012
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An internally quenched fluorogenic peptide substrate (Mca/Anb as fluorophore/quencher) that is preferentially cleaved by cathepsin G between Gnf and Ser with high efficiency and selectivity (kcat = 0.524 s-1; KM = 2.08 µM; kcat/KM = 252,000 M-1s-1) as well as sensitivity (≤70 pM Cath G; [subsrate] = 3.3 µM). Human neutrophil elastase and proteinase 3 are found to cleave the peptide between Val and Thr with a much slower kinetics (no detectable cleavage in 1 h) and lower efficiency (10% and 23% cleavage, respectively, after 24 h).
Mca = 7-Methoxycoumarin-4-yl acetyl; Gnf = 4-Guanidine-L-phenylalanine; Anb = Amino nitrobenzoic acid.
| Product information | |||
|---|---|---|---|
| Peptide Sequence | Mca-Phe-Val-Thr-Gnf~Ser-Trp-Anb-NH2 | ||
| Form | Lyophilized solid | ||
| Formulation | Supplied as a trifluoroacetate salt | ||
| Molar mass | 1222.3 | ||
| Protect from Light | Yes | ||
| Hygrocopic | Yes | ||
| Packaged under inert gas | Yes | ||
| Purity | ≥97% by HPLC | ||
| Excitation max. | 325 nm | ||
| Emission max. | 400 nm | ||
| Solubility | DMSO | ||
| Chemical formula | C61H67N13O15 | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Shipped with Blue Ice or with Dry Ice
Standard Handling |
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