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31 May 2012
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Mixture of collagenolytic proteases that can be separated and characterized by FPLC. Has high activity against different types of collagen, especially calf skin collagen Type III and bovine lens capsule collagen Type IV, that are resistant to clostridial collagenases. Excellent, general-purpose protease that has chymotrypsin-, trypsin-, and elastase-like specificity and cleaves both standard synthetic and protein substrates. Ability to cleave native, insoluble collagen makes it particularly suitable for isolation of viable cells from tissues. Recommended for harvesting of endothelial cells.
Note: 1 KU = 1000 units.
| Product information | |||
|---|---|---|---|
| Form | Lyophilized | ||
| Molar mass | 70000 | ||
| Hygrocopic | Yes | ||
| Unit definition | One Mandl unit is defined as the amount of enzyme that will release 1.0 µmol Leu equivalent from collagen in 5 h at 37°C, pH 7.5. | ||
| Activity | ≥1,000 Mandl units/mg dry weight | ||
| pH value | optimum 7.7. Stable at 6.5 - 8.5 | ||
| PI | 2.3, temperature optimum 37°C | ||
| Solubility | Aqueous Buffers | ||
| EC number | 3.4.24.3 | ||
| CAS number | 9001-12-1 | ||
| Merck USA index | 14, 2481 | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Ambient Temperature Only
Harmful |
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| Safety information | |||
|---|---|---|---|
| S Phrase |
S: 22-24-37 Do not breathe dust. Avoid contact with skin. Wear suitable gloves. |
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| R Phrase |
R: 36/37/38-42/43 Irritating to eyes, respiratory system and skin. May cause sensitization by inhalation and skin contact. |
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