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250301 Cytochrome b₅, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
250301
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      Recombinant, human cytochrome b5 expressed in E. coli. A membrane-bound hemoprotein that enhances the catalytic efficiency of some P450 isoforms, most notably members of the 3A subfamily. Serves as an electron buffer and is speculated to play either a direct role in electron transfer or an indirect role through allosteric interactions with P450. Suitable for in vitro drug metabolism and toxicology studies including reconstituted P450-dependent biotransformation reactions.
      Heme content: ≥60 nmol/mg protein.
      Catalogue Number250301
      Brand Family Calbiochem®
      References
      ReferencesHolmans, P.J., et al. 1994. Arch. Biochem. Biophys. 312, 554.
      Gillam, E.M.J., et al. 1993. Arch. Biochem. Biophys. 305, 123.
      Product Information
      CAS number9078-32-4
      FormLiquid
      FormulationIn 20 mM sodium/potassium phosphate buffer, 100 µM EDTA, pH 7.25.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      250301 0

      Documentation

      References

      Reference overview
      Holmans, P.J., et al. 1994. Arch. Biochem. Biophys. 312, 554.
      Gillam, E.M.J., et al. 1993. Arch. Biochem. Biophys. 305, 123.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-September-2007 RFH
      DescriptionRecombinant, human cytochrome b5 expressed in E. coli. A membrane-bound hemoprotein that enhances the catalytic efficiency of some cytochrome-P450 isoforms, most notably members of the 3A subfamily. Speculated to either play a direct role in electron transfer or an indirect role through allosteric interactions with cytochrome-P450. Suitable for in vitro drug metabolism and toxicology research including reconstituted P450-dependent biotransformation reactions.
      FormLiquid
      FormulationIn 20 mM sodium/potassium phosphate buffer, 100 µM EDTA, pH 7.25.
      CAS number9078-32-4
      Purity≥90% by SDS-PAGE
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesHolmans, P.J., et al. 1994. Arch. Biochem. Biophys. 312, 554.
      Gillam, E.M.J., et al. 1993. Arch. Biochem. Biophys. 305, 123.