324713
Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus
V8 Protease
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Merck KGaA
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64293 Darmstadt
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01 June 2012
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Native endoproteinase Glu-C from Staphylococcus aureus. Serine protease that specifically hydrolyzes peptide bonds at the carboxylic side of glutamic acid and aspartic acid residues. Inhibited by DFP and α2-macroglobulin. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
| Product information | |||
|---|---|---|---|
| Form | Lyophilized | ||
| Molar mass | 27000 | ||
| Specific activity | ≥15 units/mg protein | ||
| Unit definition | One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-Phe-Leu-Glu-pNA per min at 25°C, pH 7.8. | ||
| Solubility | H2O | ||
| EC number | 3.4.21.19 | ||
| CAS number | 66676-43-5 | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Ambient Temperature Only
Harmful |
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| Safety information | |||
|---|---|---|---|
| S Phrase |
S: 22-26-36 Do not breathe dust. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing. |
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| R Phrase |
R: 20-36/37/38-42/43 Harmful by inhalation. Irritating to eyes, respiratory system and skin. May cause sensitization by inhalation and skin contact. |
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