Millipore Sigma Vibrant Logo

70724 FRETWorks™ S•Tag™ Assay Kit

Ultrasensitive, homogeneous, fluorescent assay of S•Tag fusion proteins

FRETWorks™ S•Tag™ Assay Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70724
  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      The FRETWorks™ S•Tag™ Assay is a FRET-based method that enables extremely sensitive detection of S•Tag fusion proteins in minutes with a homogeneous format. The interaction of the 15–amino acid S•Tag fusion peptide with purified S-protein reconstitutes RNase activity, which is measured using the FRET ArUAA substrate. The substrate consists of a short, mixed ribo/deoxyribooligonucleotide having a fluorophore on the 5′-end and a quencher on the 3′-end (Keleman 1999). Fluorescence of the uncleaved molecule is inhibited by the interaction of the fluorophore and quencher; upon cleavage of the intervening ribonucleotide residue, the fluorophore is released and becomes highly fluorescent. The specificity of the substrate permits this assay to be performed with crude extracts. No reagent injection is necessary, and the signal is stable for > 6 hours.

      The FRETWorks S•Tag Assay Kit is compatible with the PopCulture® Reagent (including the additives Benzonase® Nuclease and rLysozyme™ Solution), making these reagents an ideal combination for rapid, automatable screening of protein expression.
      Catalogue Number70724
      Brand Family Novagen®
      References
      ReferencesKeleman, B.R., et al. 1999. Nucleic Acids Res. 27, 3696.
      Product Information
      100 assays1000 assays
      •0.5 ml 5 mlS•Tag Grade S-protein
      •0.2 ml 2 mlFRET ArUAA Substrate
      •2.5 ml 25 ml10X FRET Assay Buffer
      •2.5 ml 25 ml10X Stop Solution
      •50 µl 500 µlS•Tag Standard (0.05 pmole/µl)
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      70724 0

      Documentation

      References

      Reference overview
      Keleman, B.R., et al. 1999. Nucleic Acids Res. 27, 3696.

      Brochure

      Title
      Bulk Product Guide

      Citations

      Title
    • Gael Carney, Shu Wei and William B. Rizzo. (2004) Sjögren-Larsson syndrome: seven novel mutations in the fatty aldehyde dehydrogenase gene ALDH3A2. Human Mutation 24, 186-193.
      		•

      User Protocols

      Title
      TB251 FRETWorks™ S•Tag™ Assay Kit