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Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
01 June 2012
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This product has been discontinued.
We apologize for the inconvenience, but we do not currently have an alternative product.
A pentapeptide substrate based on the carboxyl terminus of H-Ras with a dansyl group attached to the amino terminus. Serves as a substrate for continuous fluorescent monitoring of protein-farnesyl transferase (FTase) activity (kcat = 0.5 s-1; Km = 1.4 µM) in the presence of farnesyl diphosphate. S-farnesylation of the cysteine moiety results in approximately 13-fold enhancement in fluorescence intensity (monitored at λ = 505 nm) accompanied by a shift in the emission maximum from 565 nm to 515 nm. Reported to be highly selective for FTase and is not recognized by geranylgeranyltransferase I. Useful for screening of potential FTase inhibitors. ε340 = 4,250 M-1 cm-1 in 20 mM Tris-HCI, 10 mM EDTA, pH 7.5.
| Product information | |||
|---|---|---|---|
| Form | Lyophilized | ||
| Formulation | Supplied as a trifluoroacetate salt. | ||
| Molar mass | 710.9 | ||
| Protect from Light | Yes | ||
| Hygrocopic | Yes | ||
| Packaged under inert gas | Yes | ||
| Purity | ≥95% by HPLC | ||
| Excitation max. | ~340 nm | ||
| Emission max. | ~505 nm | ||
| Solubility | DMSO | ||
| Chemical formula | C31H46N6O9S2 | ||
| CAS number | 143744-88-1 | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Standard Handling |
||
