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382176 Histone Deacetylase 3, His•Tag®, Human Recombinant, S. frugiperda

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: HDAC3, HDAC3/NcoR2, HDAC3/SMRT

382176
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      Full-length, recombinant, human histone deacetylase 3 (HDAC3) fused at the C-terminus to a His•Tag® sequence and human SMRT (NCOR2) consisting of amino acids 395-489 fused at the N-terminus to GST and co-expressed in S. frugiperda insect cells using a baculovirus expression system. SMRT co-expression is required for full HDAC3 activity. The HDAC3-SMRT repression complex represents a novel and potentially specific therapeutic target. The purified complex is useful for the study of HDAC3 regulation and for inhibitor screening.
      MW: 49700 (HDAC3) and 39000 (SMRT)
      Catalogue Number382176
      Brand Family Calbiochem®
      SynonymsHDAC3, HDAC3/NcoR2, HDAC3/SMRT
      References
      ReferencesGuenther, M.G., et al. 2000. Genes Dev. 14, 1048.
      Li, J., et al. 2000. EMBO J. 19, 4342.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enyme that is required to deacetylate 1 pmol fluorescent peptide substrate [Arg-His-Lys-Lys(Ac)], per min at 37°C.
      FormLiquid
      FormulationIn 138 mM NaCl, 25 mM Tris-HCl, 0.05% TWEEN®-20 detergent, 10% glycerol, pH 8.0.
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Specific Activity≥110 units/µg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      382176 0

      Documentation

      References

      Reference overview
      Guenther, M.G., et al. 2000. Genes Dev. 14, 1048.
      Li, J., et al. 2000. EMBO J. 19, 4342.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision29-June-2011 JSW
      SynonymsHDAC3, HDAC3/NcoR2, HDAC3/SMRT
      DescriptionFull-length, recombinant, human histone deacetylase 3 (HDAC3) fused at the C-terminus to a His•Tag® sequence and human SMRT (NCOR2) consisting of amino acids 395-489 fused at the N-terminus to GST and co-expressed in S. frugiperda insect cells using a baculovirus expression system. SMRT co-expression is required for full HDAC3 activity. The HDAC3-SMRT repression complex represents a novel and potentially specific therapeutic target. The purified complex is useful for the study of HDAC3 regulation and for inhibitor screening. MW: 49700 (HDAC3) and 39000 (SMRT).
      FormLiquid
      FormulationIn 138 mM NaCl, 25 mM Tris-HCl, 0.05% TWEEN®-20 detergent, 10% glycerol, pH 8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions
      137 mM NaCl, 25 mM Tris-HCl, 2.7 mM KCl, 1 mM MgCl2, 100 µg/ml BSA, pH 8.0, 30 µM substrate [Arg-His-Lys-Lys(Ac)], 1 ng/µl HDAC3; incubate at 37°C for 30 min.
      Purity≥90% by SDS-PAGE
      Specific activity≥110 units/µg protein
      Unit definitionOne unit is defined as the amount of enyme that is required to deacetylate 1 pmol fluorescent peptide substrate [Arg-His-Lys-Lys(Ac)], per min at 37°C.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesGuenther, M.G., et al. 2000. Genes Dev. 14, 1048.
      Li, J., et al. 2000. EMBO J. 19, 4342.