71256  MagPrep^® Bacterial Genomic DNA Kit

Efficient isolation of genomic DNA from Gram-positive and Gram-negative bacterial species

The MagPrep® Bacterial Genomic DNA Kit is designed for purification of genomic DNA from both Gram-positive and Gram-negative bacteria. This kit combines the advantages of a silica-based resin with magnetic-based separation. The method is based on the lysis of bacterial cells in proximity to magnetic silica particles, and the immobilization of large DNA molecules on the surface of the particles. The resulting DNA:magnetic particle complex is washed to remove impurities and purified genomic DNA is eluted. The procedure for Gram-negative bacteria can be performed in as little as 30 minutes, and genomic DNA from Gram-positive bacteria can be purified in as little as 75 minutes, with much of the procedure being hands-off time. Yields of up to 30 µg of genomic DNA can be achieved with 1 ml of culture. Genomic DNA prepared with MagPrep Bacterial Genomic DNA Kit is suitable for a variety of downstream applications including restriction endonuclease digestion, RFLP analysis, Southern blotting, library construction, and many PCR-based applications.

Table 1. Strains processed successfully with the MagPrep Bacterial Genomic DNA Kit
Aerococcus viridans Agrobacterium radiobacter Alcaligenes faecalis Bacillus cereus Bacillus subtilis Citrobacter freundii Enterobacter cloacae Enterococcus faecalis Enterococcus faecium Erysipelothrix rhusiopathiae Escherichia coli B Escherichia coli K-12 Klebsiella pneumoniae	Lactobacillus casei Lactococcus lactis Leuconostoc mesenteroides Listeria innocua plus 12 subspecies Micrococcus luteus Proteus vulgaris Salmonella enterica Serratia marcescens Shigella sonnei Staphylococcus aureus Staphylococcus simulans Vibrio parahemolyticus Yersinia enterocolitica