444146
MDM2, His•Tag®, Human, Recombinant, E. coli
Mouse Double Minute 2, Human, Recombinant, E. coli
For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
01 June 2012
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This product has been discontinued.
We apologize for the inconvenience, but we do not currently have an alternative product. For related products, please refer to our complete listing of proteasome and ubiquitination enzymes.
Recombinant, human MDM2 expressed in E. coli and purified by affinity chromatography using FPLC. Useful for in vitro transcription assays and DNA-protein and protein interaction assays. MDM2 is an E3 ubiquitin ligase that was first identified in mouse as a small acentromeric extrachromosomal element. It was then shown to become oncogenic upon amplification and tumorigenic when over-expressed in NIH3T3 and Rat2 cells. MDM2 overexpression is known to promote tumor development by inhibiting wild-type p53 transcriptional activation.
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 100 mM KCl, 20 mM Tris-Cl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0. | ||
| Molar mass | 54000 | ||
| Avoid freeze/thaw | Yes | ||
| Unit definition | One unit is defined as 1 ng purified protein. | ||
| Biological activity | 20-200 ng is sufficient for an in vitro protease assay and 100 ng is sufficient for a protein-protein interaction assay. | ||
| Purity | > 95% by SDS-PAGE | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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