444208
MMP-1, Proenzyme, Human Rheumatoid Synovial Fibroblast
Matrix Metalloproteinase 1, Human Interstitial Collagenase, Collagenase-1
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Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
01 June 2012
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Native proMMP-1 from cultured, human rheumatoid synovial fibroblast. Corresponds to the 56 kDa/52 kDa enzyme. May contain some activated enzyme but requires activation by APMA (Cat. No. 164610) for 30 - 60 min. at 30°C just prior to use to obtain fully activated enzyme (46 kDa/42 kDa). May also undergo autocatalysis to yield a 27 kDa/22 kDa active enzyme. Expressed by a large number of cell types. Cleaves fibrillar type I collagen.
M.W. 56,000/52,000. Note: 1 mU = 1 milliunit.
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 300 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl2, 1 µM ZnCl2, 0.05% BRIJ® 35 Detergent, 0.05% NaN3, pH 7.0. | ||
| Avoid freeze/thaw | Yes | ||
| Specific activity | ≥15 mU/mg protein | ||
| Unit definition | One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C pH 7.0. | ||
| Purity | ≥90% by SDS-PAGE | ||
| Contaminants | no other MMP activity detectable | ||
| Source | Prepared from culture medium of human rheumatoid synovial fibroblasts that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV. | ||
| EC number | 3.4.24.7 | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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