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69831  Mouse Ig-Primer Set

For general questions please contact our Customer Service:

Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000

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01 June 2012

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Product number Qty/Pk Quantity Price
69831-3  1 set 

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Prices are subject to change without notice.
The Ig-Primer Sets are designed for amplification of immunoglobulin (Ig) variable region cDNAs from human and mouse sources. Mammalian light- and heavy-chain Igs contain conserved regions adjacent to the hypervariable complementarity defining regions (CDRs). Appropriately designed oligonucleotide primer sets enable these regions to be specifically amplified using PCR (Larrick 1989, Jones 1991), after which the products may be sequenced directly or cloned. This powerful method enables rapid, precise analysis of hybridomas, myeloma cell progression and heterogeneity, idiotype networks, B-cell clonal ontogeny, and phylogenetic relationships.

In practice, total RNA is isolated from a B-cell source, such as peripheral blood, lymph node(s), spleen, or hybridoma, and first-strand cDNA is prepared using reverse transcriptase. The primer sets are then used in PCR to specifically amplify the variable regions of light- and heavy-chain cDNAs.

Please call Technical Service if you need more information regarding individual Ig-Primer Sets.


Related products
69830 Human Ig-Primer Set
Product information
Avoid freeze/thaw Yes
Components
•500 pmolMu IgM VH 3′-1 Primer
•500 pmolMu IgG VH 3′-2 Primer
•500 pmolMu Ig VH 5′-A Primer
•500 pmolMu Ig VH 5′-B Primer
•100 pmolMu Ig VH 5′-C Primer
•100 pmolMu Ig VH 5′-D Primer
•100 pmolMu Ig VH 5′-E Primer
•100 pmolMu Ig VH 5′-F Primer
•500 pmolMu Igκ VL 3′-1 Primer
•500 pmolMu Igκ VL 5′-A Primer
•500 pmolMu Igκ VL 5′-B Primer
•100 pmolMu Igκ VL 5′-C Primer
•100 pmolMu Igκ VL 5′-D Primer
•100 pmolMu Igκ VL 5′-E Primer
•100 pmolMu Igκ VL 5′-F Primer
•100 pmolMu Igκ VL 5′-G Primer
•500 pmolMu Igλ VL 3′-1 Primer
•500 pmolMu IgMλ VL 5′-A Primer
•10 µlMouse Positive Control PCR Template
Store and ship information
Storage -20°C
Ship Shipped with Blue Ice or with Dry Ice
Standard Handling

© Merck KGaA, Darmstadt, Germany, 2012


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