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481490 NGB

NGB MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 1-Nitro-3-guanidinobenzene, HCl

481490
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      A chromogenic substrate for direct and continuous assay of arginase (Km = 1.6 mM, kcat = 0.09 min-1) with a detection limit of ~1 µg/ml. Arginase hydrolyzes NGB to yield urea plus the chromophore m-nitroaniline (λ = 372 nm, ε = 1,280 M-1cm-1). Although the kcat value for NGB hydrolysis is much lower than arginine, the Km value (1.6 mM) is nearly identical to arginine (Km = 1.4 mM).
      Catalogue Number481490
      Brand Family Calbiochem®
      Synonyms1-Nitro-3-guanidinobenzene, HCl
      References
      ReferencesBaggio, R., et al. 1999. Anal. Biochem. 276, 251.
      Product Information
      CAS number24067-13-8
      FormOff-white solid
      Hill FormulaC₇H₈N₄O₂ · HCl
      Chemical formulaC₇H₈N₄O₂ · HCl
      Hygroscopic Hygroscopic
      Structure formula ImageStructure formula Image
      Applications
      Biological Information
      Purity≥97% by HPLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 1 month at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      481490 0

      Documentation

      References

      Reference overview
      Baggio, R., et al. 1999. Anal. Biochem. 276, 251.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-April-2011 RFH
      Synonyms1-Nitro-3-guanidinobenzene, HCl
      DescriptionA chromogenic substrate for direct and continuous assay of arginase (Km = 1.6 µM, kcat = 0.09 min-1) with a detection limit of ~1 µg/ml. Upon hydrolysis by arginase, NGB yields urea plus the chromophore m-nitroaniline (λ = 372 nm, ε = 1,280 M-1cm-1). Although the kcat value for NGB hydrolysis is much lower than arginine, the Km values are nearly identical to arginine (Km = 1.4 µM).
      FormOff-white solid
      Intert gas (Yes/No) Packaged under inert gas
      CAS number24067-13-8
      Chemical formulaC₇H₈N₄O₂ · HCl
      Structure formulaStructure formula
      Purity≥97% by HPLC
      SolubilityDMSO (25 mg/ml)
      Storage Protect from light
      -20°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 1 month at -20°C.
      Toxicity Standard Handling
      ReferencesBaggio, R., et al. 1999. Anal. Biochem. 276, 251.