For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
01 June 2012
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pBAC™-3 is a baculovirus transfer plasmid compatible with BacVector™-1000, -2000, or -3000 Triple Cut Virus DNA for low background transfection and efficient utilization of the polh promoter and expression of secreted proteins. A 20 amino acid gp64 signal peptide sequence is capable of directing high levels of protein into the secretory pathway of infected insect cells. Cloning sites are provided for the creation of N-terminal fusions of an insert with His•Tag® and/or S•Tag™ sequences. pBAC-3 provides an ATG start codon at the optimal position relative to native polyhedrin translation signals. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Single stranded sequencing of phage-derived DNA can be performed using the 1629DWN primer.
| Related products | |
|---|---|
| 70003 | pBAC™-1 DNA |
| 70004 | pBAC™-2cp DNA |
| 70049 | pBACgus-2cp DNA |
| 70054 | pBAC™gus-1 DNA |
| Related products for pBAC™-3 DNA | |
| 70003 | pBAC™-1 DNA |
| 70004 | pBAC™-2cp DNA |
| 70049 | pBACgus-2cp DNA |
| 70054 | pBAC™gus-1 DNA |
| 70089 | pBACgus-3 DNA |
| Product information | |||
|---|---|---|---|
| Avoid freeze/thaw | Yes | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Shipped with Blue Ice or with Dry Ice
Standard Handling |
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