70054  pBAC™gus-1 DNA

pBACgus-1 is a baculovirus transfer plasmid (Cat. No. 70054-3) designed for simplified cloning and expression of target genes in insect cells. The plasmid is compatible with BacVector™-1000, -2000 or -3000 Triple Cut Virus DNA for low background transfection and efficient utilization of the polh promoter. The plasmid also carries the gus gene encoding β-glucuronidase under control of the late basic protein promoter (P6.9), which serves as a reporter to verify recombinant viruses by staining with X-gluc. Inserts in pBACgus-1 must contain their own translation initiation codon. The vector provides optional expression of a C-terminal His•Tag^® fusion sequence by allowing read-through of inserts in the proper reading frame. Unique restriction sites are indicated on the circle map. The cloning/expression region of the coding strand transcribed from the polh promoter is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Single stranded sequencing of phage-derived DNA can be performed using the 1629DWN primer.