69965
Perfect Protein™ AP Western Blot Kit
Accurate size markers detectable on any Western blot
For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
01 June 2012
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The Perfect Protein™ AP Western Blot Kit includes an S-protein AP Conjugate in addition to the Perfect Protein™ Western Markers (Cat. No. 69959). For a version containing prestained proteins for tracking electrophoresis and Western transfer, please refer to our complete listing of Trail Mix™ Western Markers and Blot Kits.
A.Two parallel blots were incubated with primary antibodies for an expressed protein, then incubated with Anti-Mouse IgG AP Conjugate and processed using chemiluminescent detection. The S-protein AP Conjugate was included with secondary antibody for the blot on the left. Lanes: 1, 5 µl Perfect Protein Western Markers; 2, 10 ng induced cell extract; 3, 1 µg uninduced cell extract.
B.Blot contains the Perfect Protein Western Markers plus purified GUS expressed in insect cells incubated with a GUS-specific monoclonal antibody, then incubated with Anti-Mouse IgG HRP Conjugate and S-protein HRP Conjugate and detected with chemiluminescent HRP substrate. Lanes: 1, 5 µl Perfect Protein Western Markers; 2, 800 ng purified GUS protein.
A.Two parallel blots were incubated with primary antibodies for an expressed protein, then incubated with Anti-Mouse IgG AP Conjugate and processed using chemiluminescent detection. The S-protein AP Conjugate was included with secondary antibody for the blot on the left. Lanes: 1, 5 µl Perfect Protein Western Markers; 2, 10 ng induced cell extract; 3, 1 µg uninduced cell extract.
B.Blot contains the Perfect Protein Western Markers plus purified GUS expressed in insect cells incubated with a GUS-specific monoclonal antibody, then incubated with Anti-Mouse IgG HRP Conjugate and S-protein HRP Conjugate and detected with chemiluminescent HRP substrate. Lanes: 1, 5 µl Perfect Protein Western Markers; 2, 800 ng purified GUS protein.
| Related products | |
|---|---|
| 69078 | Perfect Protein™ HRP Western Blot Kit |
| 69959 | Perfect Protein™ Western Markers |
| Related products for Perfect Protein™ AP Western Blot Kit | |
| 69078 | Perfect Protein™ HRP Western Blot Kit |
| 69959 | Perfect Protein™ Western Markers |
| Product information | |||||||||
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| Form | Liquid | ||||||||
| Avoid freeze/thaw | Yes | ||||||||
| Components |
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| Features and benefits |
• Detect markers simply by adding the S-protein (AP or HRP Conjugate) or His•Tag® Monoclonal Antibody to the same incubation used for sample detection. • S-protein conjugate (included in kits) does not interfere with antibodies or streptavidin detection. • Can be used with colorimetric and chemiluminescent AP or HRP substrates. • Recombinant, unmodified markers give sharp, accurately sized bands. • Markers are supplied at the working dilution in gel loading buffer; concentration optimized for Western detection. • Protein sizes are 15, 25, 35, 50, 75, 100, and 150 kDa. |
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| Store and ship information | |||
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| Storage | -20°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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