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71731  pIEx/Bac™-3 3C/LIC Vector Kit

For general questions please contact our Customer Service:

Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000

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01 June 2012

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Product number Qty/Pk Quantity Price
71731-3  20 rxn  loading
Prices are subject to change without notice.
The pIEx/Bac™-3 3C/LIC vector is a 3C/LIC prepared version of pIEx/Bac-3. The pIEx/Bac™ vectors are dual-purpose vectors that offer unique flexibility for cloning and expression of proteins in Spodoptera-derived insect cells. These vectors contain the Autographa californica nuclear polyhedrosis virus (AcNPV) enhancer/immediate early promoter combination (hr5/ie1) for plasmid-mediated and early baculovirus expression, and the AcNPV p10 very late promoter for robust baculovirus-mediated expression. All pIEx/Bac plasmids carry AcNPV-derived virus regions to mediate recombination at the polh locus. The pIEx/Bac-3 vector contains an N-terminal His•Tag® coding sequence with 10 histidines followed by an HRV 3C protease cleavage site and a multiple cloning site, with the option to include a C-terminal thrombin protease cleavage site and 8 aa Strep•Tag® II coding sequence.
Product information
Components
20 µg (40 µl) 0.5 µg/µl pIEx/Bac-3 3C/LIC DNA
50 µlT4 DNA Polymerase Buffer
5 µg (10 µl) 0.5 µg/µl pIEx/Bac 3C/LIC Positive Expression Control
25 UT4 DNA Polymerase, LIC-qualified
100 µl100 mM DTT
40 µl25 mM dATP
50 µl25 mM EDTA
1.5 ml Nuclease-free Water
1 pkgNovaBlue GigaSingles Competent Cells
2 mlSOC Medium
10 µl Test Plasmid
Store and ship information
Storage ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS

© Merck KGaA, Darmstadt, Germany, 2012


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