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The pIEx/Bac™-3 3C/LIC vector is a 3C/LIC prepared version of pIEx/Bac-3. The pIEx/Bac™ vectors are dual-purpose vectors that offer unique flexibility for cloning and expression of proteins in Spodoptera-derived insect cells. These vectors contain the Autographa californica nuclear polyhedrosis virus (AcNPV) enhancer/immediate early promoter combination (hr5/ie1) for plasmid-mediated and early baculovirus expression, and the AcNPV p10 very late promoter for robust baculovirus-mediated expression. All pIEx/Bac plasmids carry AcNPV-derived virus regions to mediate recombination at the polh locus. The pIEx/Bac-3 vector contains an N-terminal His•Tag® coding sequence with 10 histidines followed by an HRV 3C protease cleavage site and a multiple cloning site, with the option to include a C-terminal thrombin protease cleavage site and 8 aa Strep•Tag® II coding sequence.
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Product information
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Components
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| • | 20 µg (40 µl) | 0.5 µg/µl pIEx/Bac-3 3C/LIC DNA | | • | 50 µl | T4 DNA Polymerase Buffer | | • | 5 µg (10 µl) | 0.5 µg/µl pIEx/Bac 3C/LIC Positive Expression Control | | • | 25 U | T4 DNA Polymerase, LIC-qualified | | • | 100 µl | 100 mM DTT | | • | 40 µl | 25 mM dATP | | • | 50 µl | 25 mM EDTA | | • | 1.5 ml | Nuclease-free Water | | • | 1 pkg | NovaBlue GigaSingles Competent Cells | | • | 2 ml | SOC Medium | | • | 10 µl | Test Plasmid | |
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Store and ship information
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Storage
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≤ -70°C
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Ship
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Dry Ice Only
Multiple Toxicity Values, refer to MSDS
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