For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
01 June 2012
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The pIEx/Bac™-5 3C/LIC vector carries an N-terminal GST•Tag™ coding sequence followed by a recognition site for the human rhinovirus (HRV) 3C protease. This protease is highly specific for cleavage of the sequence LEVLFQ_GP, and is active at low temperatures. The 3C/LIC cloning site is followed by an optional C-terminal thrombin recognition site and a Strep•Tag® II coding sequence. This 3C/LIC vector is ready for rapid, directional cloning of PCR-amplified DNA, without restriction enzyme digestion or ligation. The pIEx/Bac vectors are dual-purpose vectors that offer unique flexibility for cloning and expression of proteins in Spodoptera-derived insect cells. These vectors contain the Autographa californica nuclear polyhedrosis virus (AcNPV) enhancer/immediate early promoter combination (hr5/ie1) for plasmid-mediated and early baculovirus expression, and the AcNPV p10 very late promoter for robust baculovirus-mediated expression. All pIEx/Bac plasmids carry AcNPV-derived virus regions to mediate recombination at the polh locus.
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| Storage | ≤ -70°C | ||
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Dry Ice Only
Harmful |
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| Safety information | |||
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| S Phrase |
S: 26-36 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing. |
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| R Phrase |
R: 22-36/37/38 Harmful if swallowed. Irritating to eyes, respiratory system and skin. |
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