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01 junio 2012
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The ProteoExtract® Phosphopeptide TiO2 Enrichment Kit is a useful tool for enrichment of phosphorylated peptides from complex samples by a highly selective Titanium oxide solid phase. It enhances the ability to routinely identify and characterize large numbers of phosphorylated species within complex protein mixtures taking advantage of a novel titanium dioxide material. Captured and eluted phosphopeptide fractions are ready for MALDI- and ESI- mass spectrometry analysis.
| Información sobre producto | |||
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| Format | Phosphopeptide enrichment | ||
| Form | 100 Isolations | ||
| Detection method | Mass Spectrometry | ||
| Kit contains | TiO2 Phosphobind Resin, 10X TiO2 Phosphobind Buffer, Wash Buffer 1, Wash Buffer 2, TiO2 Elution Buffer, Dihydroxybenzoic Acid, and a user protocol. | ||
| Almacenar y enviar información | |||
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Advertencia de materiales peligrosos: Debido a la naturaleza de los materiales peligrosos de este envío, podrían aplicarse gastos de envío añadidos a su pedido. Ciertos tamaños pueden estar exentos de los gastos de envío añadidos a los materiales peligrosos. Consulte a su oficina local de ventas si desea más información relativa a esos gastos. |
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| Categoría de alamacenamiento | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Datos | |||
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 <b>Titanium dioxide allows for selective and sensitive phosphopeptide enrichment with low background from complex mixtures.</b> A complex peptide mixture derived from a tryptic digest of porcine liver extract was spiked with α-casein and two synthetic phosphopeptides (see below for concentrations). The samples were subsequently processed using the ProteoExtract<sup>®</sup> Phosphopeptide Enrichment TiO<sub>2</sub> Kit as outlined in the Detailed Protocol. Mass spectrometry analysis was performed using ESI-LC/MS equipment operated in positive mode. (A) Unprocessed sample (B) Phosphopeptides recovered using the ProteoExtract<sup>®</sup> Phosphopeptide Enrichment TiO<sub>2</sub> Kit. <b>Spiked Phosphopeptides:</b> 1. 50 nM Angiotensin ~6 pmol/125 µl; ~7 ng/125 µl 2. 67 nM Calcineurin Substrate ~8 pmol/125 µl; ~18 ng/125 µl 3. 140 nM α-Casein ~18 pmol/125 µl; ~400 ng/125 µl |
