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01 Juni 2012
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pRSFDuet™-1 is designed for the coexpression of two target proteins. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). The vector also carries the pRSF1030 replicon (also known as NTP1), lacI gene, and kanamycin resistance marker. This vector can be transformed into the same cell with pETDuet™-1, pACYCDuet™-1, and/or pCDFDuet™-1 for the coexpression of up to eight target proteins. ORFs inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. ORFs inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer.
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