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71228  pSTBlue-1 AccepTor™ Vector Giga Kit

Featuring super-efficient competent cells for increased transformation efficiency

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01 junio 2012

cargando
 
Número de producto Cant./Env. Cantidad Precio
71228-3  20 rxn  cargando
71228-4  40 rxn  cargando
Los precios están sujetos a cambios sin notificación.

The pSTBlue-1 AccepTor™ Vector Giga Kits and pSTBlue-1 Perfectly Blunt® Giga Cloning Kits feature NovaBlue GigaSingles™ Competent Cells for increased transformation efficiency. AccepTor Vector Kits are designed for the cloning of PCR products generated using thermostable DNA polymerases that leave single 3′-dA nucleotide overhangs. The vector enables direct ligation by virtue of single 3′-dU overhangs that anneal with 3′-dA extensions on PCR products. The dU residues are converted to dT residues in vivo.

The Perfectly Blunt Cloning Kits are designed for the cloning of DNA generated by PCR using any type of DNA polymerase. This allows for the use of high-fidelity proofreading enzymes for amplification, thereby decreasing the probability of generating mutations in the target sequence.


Información sobre producto
Components
2 or 4 × 0.5 µgpSTBlue-1 AccepTor Vector
10 µlAccepTor™ Vector Positive Control Insert
2 or 4 × 55 µlClonables™ 2X Ligation Premix
1.5 mlNuclease-free Water
22 or 44 × 50 µlNovaBlue GigaSingles™ Competent Cells
4 or 7 × 2 mlSOC Medium
10 µlTest Plasmid
Features and benefits • No restriction enzymes or special primers
• Direct ligation of PCR product with vector
• Compatible with polymerases that leave single 3´-dA overhangs
• Blue/white screening with pSTBlue-1 or pETBlue™-1 vectors
• Simple protocol takes as little as 40 minutes from PCR product to plating transformants
Declaration This product is covered by U.S. Patent 5,856,144 owned by EMD Chemicals Inc. or its Affiliates.
Almacenar y enviar información
Categoría de alamacenamiento ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS

© Merck KGaA, Darmstadt, Alemania, 2012


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