For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
01 June 2012
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The pT7Blue-2 vector features the pUC19 backbone (including high-copy number origin of replication and lac sequences), f1 origin of replication and modified multiple cloning region designed for optimal expression of cloned inserts by in vitro translation. The multiple cloning region contains a T7 promoter upstream of the Xenopus β-globin 5′ UTR translation enhancer and S•Tag™ sequences, followed by an enterokinase cleavage site and extensive region of cloning sites. An SP6 promoter is placed within the multiple cloning sites to allow the synthesis of essentially unfused RNA from DNA inserted downstream.
| Related products | |
|---|---|
| 70185 | Introductory pT7Blue-2 Perfectly Blunt® Cloning Kit |
| 70190 | pT7Blue-2 Perfectly Blunt® Cloning Kit |
| Related products for pT7Blue-2 Blunt Vector (linearized) | |
| 70185 | Introductory pT7Blue-2 Perfectly Blunt® Cloning Kit |
| 70190 | pT7Blue-2 Perfectly Blunt® Cloning Kit |
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| Avoid freeze/thaw | Yes | |||||||||||||||||
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| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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