71576  pTriEx™-6 3C/LIC Vector Kit

Directional PCR cloning into a multisystem expression vector

The pTriEx™-6 3C/LIC vector is a 3C/LIC version of pTriEx-6. The pTriEx-6 3C/LIC vector is uniquely designed to allow rapid characterization of target genes in multiple expression systems. With this vector, a single recombinant plasmid can be used to test expression in E. coli, insect, and mammalian cells. Transient mammalian expression is mediated by the CMV immediate early enhancer and promoter. For expression in insect cells, the pTriEx-6 3C/LIC vector contains flanking baculovirus sequences to permit the generation of recombinant baculoviruses using the BacVector® System or BacMagic™ Kits. In baculovirus-infected insect cells, expression is driven by the very late p10 promoter. Expression in E. coli is regulated by the tightly controlled T7lac promoter. The pTriEx-6 3C/LIC vector contains an N-terminal 8 aa Strep•Tag® II coding sequence followed by a HRV 3C protease cleavage site and a multiple cloning site, with the option to encode optional sequences for a C-terminal thrombin cleavage site and a His•Tag® (10-histidine) fusion.

* Strep•Tag® technology for protein purification and detection is covered under US Patent No. 5,506,121, UK Patent No. 2,272,698 and French Patent No. 93 13 066. Strep•Tactin® is covered by US Patent No. 6,103,493. These products are provided only for research use. Information about licenses for commercial use is available from IBA GmbH, Rudolf-Wissell-Str. 28, D-37079 Goettingen, Germany.

This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.